Graphene immobilized enzyme/polyethersulfone mixed matrix membrane: Enhanced antibacterial, permeable and mechanical properties
MetadataShow full item record
© 2015 Elsevier B.V. All rights reserved. Enzyme immobilization has been developed to address lots of issues of free enzyme, such as instability, low activity and difficult to retain. In this study, graphene was used as an ideal carrier for lysozyme immobilization, including graphene oxide (GO) immobilized lysozyme (GO-Ly) and chemically reduced graphene oxide (CRGO) immobilized lysozyme (CRGO-Ly). Herein, lysozyme as a bio-antibacterial agent has excellent antibacterial performance and the products of its catalysis are safety and nontoxic. Then the immobilized lysozyme materials were blended into polyethersulfone (PES) casting solution to prepare PES ultrafiltration membrane via phase inversion method. GO and CRGO were characterized by Fourier transform infrared spectroscopy (FTIR), Ultraviolet-visible spectrum (UV), X-ray diffraction (XRD), and transmission electron microscopy (TEM) and the immobilized lysozyme composites were observed by fluorescent microscopy. The results revealed that GO and CRGO were successfully synthesized and lysozyme was immobilized on their surfaces. The morphology, hydrophilicity, mechanical properties, separation properties and antibacterial activity of the hybrid membranes were characterized in detail. The hydrophilicity, water flux and mechanical strength of the hybrid membranes were significantly enhanced after adding the immobilized lysozyme. In the antibacterial experiment, the hybrid membranes exhibited an effective antibacterial performance against Escherichia coli (E. coli).
Showing items related by title, author, creator and subject.
Development of a novel polyethersulfone ultrafiltration membrane with antibacterial activity and high flux containing halloysite nanotubes loaded with lysozymeZhao, Q.; Liu, C.; Liu, Jian; Zhang, Y. (2015)In this study, halloysite nanotubes (HNTs) were used to immobilize lysozyme via a covalent binding reaction. Immobilized lysozyme (HNTs-Ly) was then added to a polyethersulfone (PES) polymer solution to prepare hybrid ...
Wang, J.; Qin, L.; Lin, J.; Zhu, J.; Zhang, Y.; Liu, Jian; Van der Bruggen, B. (2017)© 2017 Elsevier B.V. This work is focused on the application of a novel antibacterial nanofiltration (NF) membrane formed by interfacial polymerization on a polyethersulfone supporting membrane for dye removal. Lysozyme, ...
In situ formation of Ag nanoparticles in PVDF ultrafiltration membrane to mitigate organic and bacterial foulingLi, Xin Yong; Pang, R.; Li, J.; Sun, X.; Shen, J.; Han, W.; Wang, L. (2013)Membrane fouling remains one of the most problematic issues surrounding membrane use in water and wastewater treatment. Organic fouling and biological fouling contribute to irreversible fouling and flux decline in these ...