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dc.contributor.authorRowlands, J.
dc.contributor.authorWalz, N.
dc.contributor.authorRowles, J.
dc.contributor.authorKeane, Kevin
dc.contributor.authorCarlessi, Rodrigo
dc.contributor.authorNewsholme, P.
dc.date.accessioned2019-02-19T04:15:40Z
dc.date.available2019-02-19T04:15:40Z
dc.date.created2019-02-19T03:58:31Z
dc.date.issued2019
dc.identifier.citationRowlands, J. and Walz, N. and Rowles, J. and Keane, K. and Carlessi, R. and Newsholme, P. 2019. Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes. Methods in Molecular Biology. 1916: pp. 329-340.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/74054
dc.identifier.doi10.1007/978-1-4939-8994-2_32
dc.description.abstract

In type 2 diabetes, prolonged dysregulation of signalling and ß-cell metabolic control leads to ß-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate ß-cell models enables a systematic approach to understand the impact of perturbations to the biological system. The BRIN-BD11 ß-cell line is a useful, pre-clinical cell model for ß-cell dysfunction associated with type 2 diabetes, among other metabolic disorders. The present chapter describes detection and analysis of ß-cell dysfunction with respect to changes in bioenergetics and metabolism, generation of intracellular reactive oxygen species, and acute and chronic insulin secretion in the BRIN-BD11 cell line.

dc.publisherHumana Press
dc.titleMethod Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes
dc.typeJournal Article
dcterms.source.volume1916
dcterms.source.startPage329
dcterms.source.endPage340
dcterms.source.issn1064-3745
dcterms.source.titleMethods in Molecular Biology
curtin.departmentSchool of Pharmacy and Biomedical Sciences
curtin.accessStatusFulltext not available


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