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Molecular characterisation of the NAM-1 genes in bread wheat in Australia

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Authors
Yang, R.
Juhasz, A.
Zhang, Y.
Chen, X.
Zhang, Y.
She, M.
Zhang, J.
Maddern, R.
Edwards, I.
Diepeveen, Dean
Islam, S.
Ma, W.
Date
2018
Type
Journal Article

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Yang, R. and Juhasz, A. and Zhang, Y. and Chen, X. and Zhang, Y. and She, M. and Zhang, J. et al. 2018. Molecular characterisation of the NAM-1 genes in bread wheat in Australia. Crop and Pasture Science. 69 (12): pp. 1173-1181.
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Crop and Pasture Science
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Centre for Crop and Disease Management (CCDM)
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Abstract

The wheat NAM-B1 and NAM-A1 genes are positively associated with grain protein content (GPC) in wheat. We conducted molecular characterisation of the NAM-1 genes in 51 Australian wheat varieties (Triticum aestivum L.), with the aim of improving GPC and nitrogen-usage efficiency in Australian wheat. In summary, the wild type NAM-B1 gene, which originated from Israel, was identified in two Australian wheat varieties. Five varieties contained a deletion allele, whereas the majority (43) harboured a non-functional NAM-B1 allele and one variety contained both functional and non-functional alleles. Twenty-six Australian wheat varieties contained the NAM-A1a haplotype, which was similar to its well-characterised homoeolog NAM-B1 wild type and associated with high GPC. The NAM-D1 gene in the 51 wheat varieties was also characterised, and no gene variation in the exon regions was noted; only two single-nucleotide polymorphisms in introns 1 and 2 were found among the 51 varieties.