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Nucleic acid extraction from biomining microorganisms

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Authors
Zammit, Carla
Mutch, Lesley
Watling, H.R.
Watkin, Elizabeth
Date
2009
Type
Conference Paper

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Citation
Zammit, C.M. and Mutch, L.A. and Watling, H.R. and Watkin, E.L.J. 2009. Nucleic acid extraction from biomining microorganisms, in AMR Editors and Editorial Board (ed), 18th International Biohydrometallurgy Symposium, Sep 13 2009, pp. 159-162.Bariloche, Argentina: Trans Tech Publications
Source Title
Biohydrometallurgy 2009 (Advanced Materials Research Vol 71 -73)
Source Conference
18th International Biohydrometallurgy Symposium
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Faculty
Faculty of Health Sciences
School of Biomedical Science
School
School of Biomedical Sciences
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Abstract

Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.