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dc.contributor.authorZammit, Carla
dc.contributor.authorMutch, Lesley
dc.contributor.authorWatling, H.R.
dc.contributor.authorWatkin, Elizabeth
dc.contributor.editorAMR Editors and Editorial Board
dc.identifier.citationZammit, C.M. and Mutch, L.A. and Watling, H.R. and Watkin, E.L.J. 2009. Nucleic acid extraction from biomining microorganisms, in AMR Editors and Editorial Board (ed), 18th International Biohydrometallurgy Symposium, Sep 13 2009, pp. 159-162.Bariloche, Argentina: Trans Tech Publications

Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.

dc.publisherTrans Tech Publications
dc.subjectRNA extraction
dc.subjectDNA extraction
dc.subjectMolecular Methods
dc.titleNucleic acid extraction from biomining microorganisms
dc.typeConference Paper
dcterms.source.titleBiohydrometallurgy 2009 (Advanced Materials Research Vol 71 -73)
dcterms.source.seriesBiohydrometallurgy 2009 (Advanced Materials Research Vol 71 -73)
dcterms.source.conference18th International Biohydrometallurgy Symposium
dcterms.source.conference-start-dateSep 13 2009
dcterms.source.conferencelocationBariloche, Argentina
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available
curtin.facultyFaculty of Health Sciences
curtin.facultySchool of Biomedical Science

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