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dc.contributor.authorRamsay, Joshua
dc.contributor.authorKwong, S.
dc.contributor.authorMurphy, Riley
dc.contributor.authorYui Eto, Karina
dc.contributor.authorPrice, Karina
dc.contributor.authorNguyen, Quang
dc.contributor.authorO'Brien, Frances
dc.contributor.authorGrubb, Warren
dc.contributor.authorCoombs, Geoffrey
dc.contributor.authorFirth, N.
dc.date.accessioned2019-05-07T01:14:37Z
dc.date.available2019-05-07T01:14:37Z
dc.date.issued2016
dc.identifier.citationRamsay, J.P. and Kwon, S.M. and Murphy, J.T. and Yui Eto, K. and Price, K.J. and Nguyen, Q.T. and O'Brien, F.G. and Grubb, W.B. and Coombs, G.W. and Firth, N. 2016. An updated view of plasmid conjugation and mobilisation in Staphylococcus. Mobile Genetic Elements. 6 (4): e1208317.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/75433
dc.identifier.doi10.1080/2159256X.2016.1208317
dc.description.abstract

The horizontal gene transfer facilitated by mobile genetic elements impacts almost all areas of bacterial evolution, including the accretion and dissemination of antimicrobial-resistance genes in the human and animal pathogen Staphylococcus aureus. Genome surveys of staphylococcal plasmids have revealed an unexpected paucity of conjugation and mobilization loci, perhaps suggesting that conjugation plays only a minor role in the evolution of this genus. In this letter we present the DNA sequences of historically documented staphylococcal conjugative plasmids and highlight that at least 3 distinct and widely distributed families of conjugative plasmids currently contribute to the dissemination of antimicrobial resistance in Staphylococcus. We also review the recently documented "relaxase-in trans" mechanism of conjugative mobilization facilitated by conjugative plasmids pWBG749 and pSK41, and discuss how this may facilitate the horizontal transmission of around 90% of plasmids that were previously considered non-mobilizable. Finally, we enumerate unique sequenced S. aureus plasmids with a potential mechanism of mobilization and predict that at least 80% of all non-conjugative S. aureus plasmids are mobilizable by at least one mechanism. We suggest that a greater research focus on the molecular biology of conjugation is essential if we are to recognize gene-transfer mechanisms from our increasingly in silico analyses.

dc.languageeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.subjectMRSA
dc.subjectMob
dc.subjectantibiotic resistance
dc.subjecthorizontal gene transfer
dc.subjectmating pore
dc.subjectmobilization
dc.subjectpGO1
dc.subjectpSK41
dc.subjectplasmid
dc.subjectrelaxase
dc.subjecttype IV secretion
dc.titleAn updated view of plasmid conjugation and mobilisation in Staphylococcus
dc.typeJournal Article
dcterms.source.volume6
dcterms.source.number4
dcterms.source.startPage1
dcterms.source.endPage1
dcterms.source.issn2159-2543
dcterms.source.titleMobile Genetic Elements
dc.date.updated2019-05-07T01:14:37Z
curtin.departmentSchool of Pharmacy and Biomedical Sciences
curtin.accessStatusOpen access
curtin.facultyFaculty of Health Sciences
dcterms.source.eissn2159-256X


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