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dc.contributor.authorDorji, Dorji
dc.contributor.authorGraham, Ross
dc.contributor.authorSingh, Abhishek
dc.contributor.authorRamsay, Joshua
dc.contributor.authorPrice, Patricia
dc.contributor.authorLee, Silvia
dc.date.accessioned2019-05-07T01:25:09Z
dc.date.available2019-05-07T01:25:09Z
dc.date.issued2019
dc.identifier.citationDorji, D. and Graham, R.M. and Singh, A.K. and Ramsay, J.P. and Price, P. and Lee, S. 2019. Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model. Cellular Immunology. 337: pp. 42-47.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/75434
dc.identifier.doi10.1016/j.cellimm.2019.01.006
dc.description.abstract

© 2019 Elsevier Inc. The resurgence of whooping cough reflects novel genetic variants of Bordetella pertussis and inadequate protection conferred by current acellular vaccines (aP). Biofilm is a source of novel vaccine candidates, including membrane protein assembly factor (BamB) and lipopolysaccharide assembly protein (LptD). Responses of BALB/c mice to candidate vaccines included IFN-γ and IL-17a production by spleen and lymph node cells, and serum IgG1 and IgG2a reactive with whole bacteria or aP. Protection was determined using bacterial cultured from lungs of vaccinated mice challenged with virulent B. pertussis. Mice vaccinated with biofilm produced efficient IFN-γ responses and more IL-17a and IgG2a than mice vaccinated with planktonic cells, aP or adjuvant alone. Vaccination with aP produced abundant IgG1 with little IgG2a. Mice vaccinated with aP plus BamB and LptD retained lower bacterial loads than mice vaccinated with aP alone. Whooping cough vaccines formulated with biofilm antigens, including BamB and LptD, may have clinical value.

dc.languageEnglish
dc.publisherAcademic Press
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectCell Biology
dc.subjectImmunology
dc.subjectBordetella pertussis vaccine
dc.subjectBiofilm
dc.subjectBiofilm-associated proteins
dc.subjectWhooping cough
dc.subjectWHOLE-CELL
dc.subjectVACCINE
dc.subjectRESPONSES
dc.subjectPERTACTIN
dc.subjectSTRAINS
dc.subjectTOXIN
dc.subjectTH1
dc.titleImmunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model
dc.typeJournal Article
dcterms.source.volume337
dcterms.source.startPage42
dcterms.source.endPage47
dcterms.source.issn0008-8749
dcterms.source.titleCellular Immunology
dc.date.updated2019-05-07T01:25:07Z
curtin.departmentSchool of Pharmacy and Biomedical Sciences
curtin.accessStatusFulltext not available
curtin.facultyFaculty of Health Sciences
dcterms.source.eissn1090-2163
dc.date.embargoEnd2020-01-30


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