Exercise-induced immunosuppression: roles of reactive oxygen species and 5′-AMP-activated protein kinase dephosphorylation within immune cells
dc.contributor.author | Moir, H. | |
dc.contributor.author | Hughes, M. | |
dc.contributor.author | Potter, S. | |
dc.contributor.author | Sims, Craig | |
dc.contributor.author | Butcher, L. | |
dc.contributor.author | Davies, N. | |
dc.contributor.author | Verheggen, K. | |
dc.contributor.author | Jones, K. | |
dc.contributor.author | Thomas, A. | |
dc.contributor.author | Webb, R. | |
dc.date.accessioned | 2019-10-15T03:45:14Z | |
dc.date.available | 2019-10-15T03:45:14Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Moir, H. and Hughes, M.G. and Potter, S. and Sims, C. and Butcher, L.R. and Davies, N.A. and Verheggen, K. et al. 2010. Exercise-induced immunosuppression: roles of reactive oxygen species and 5′-AMP-activated protein kinase dephosphorylation within immune cells. Journal of Applied Physiology. 108 (5): pp. 1284-1292. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/76560 | |
dc.identifier.doi | 10.1152/japplphysiol.00737.2009 | |
dc.description.abstract |
We previously proposed 5′-AMP-activated protein kinase (AMPK) dephosphorylation within immune cells as an intracellular mechanism linking exercise and immunosuppression. In this study, AMPK phosphorylation underwent transient (<1 h) decreases (53.8 ± 7.2% basal) immediately after exercise (45 min of cycling at 70% V̇o2max) in a cohort of 16 adult male participants. Similar effects were seen with running. However, because exercise-induced inactivation of AMPK was previously shown to occur in an AMP-independent manner, the means by which AMPK is inactivated in this context is not yet clear. To investigate the hypothesis that exercise-induced inactivation of AMPK is mediated via signaling mechanisms distinct from changes in cellular AMP-to-ATP ratios, reactive oxygen species (ROS) and intracellular Ca2+ signaling were investigated in mononuclear cells before and after exercise and in cultured monocytic MM6 cells. In in vitro studies, treatment with an antioxidant (ascorbic acid, 4 h, 50 μM) decreased MM6 cell intracellular ROS levels (88.0 ± 5.2% basal) and induced dephosphorylation of AMPK (44.7 ± 17.6% basal). By analogy, the fact that exercise decreased mononuclear cell ROS content (32.8 ± 16.6% basal), possibly due to downregulation (43.4 ± 8.0% basal) of mRNA for NOX2, the catalytic subunit of the cytoplasmic ROS-generating enzyme NADPH oxidase, may provide an explanation for the AMPK-dephosphorylating effect of exercise. In contrast, exercise-induced Ca2+ signaling events did not seem to be coupled to changes in AMPK activity. Thus we propose that the exercise-induced decreases in both intracellular ROS and AMPK phosphorylation seen in this study constitute evidence supporting a role for ROS in controlling AMPK, and hence immune function, in the context of exercise-induced immunosuppression. | |
dc.language | English | |
dc.publisher | American Physiological Society | |
dc.subject | 1101 - Medical Biochemistry and Metabolomics | |
dc.title | Exercise-induced immunosuppression: roles of reactive oxygen species and 5′-AMP-activated protein kinase dephosphorylation within immune cells | |
dc.type | Journal Article | |
dcterms.source.volume | 108 | |
dcterms.source.number | 5 | |
dcterms.source.startPage | 1284 | |
dcterms.source.endPage | 1292 | |
dcterms.source.issn | 8750-7587 | |
dcterms.source.title | Journal of Applied Physiology | |
dc.date.updated | 2019-10-15T03:45:14Z | |
curtin.department | School of Education | |
curtin.accessStatus | Fulltext not available | |
curtin.faculty | Faculty of Humanities |
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