Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus
dc.contributor.author | Jenny, R.A. | |
dc.contributor.author | Hirst, C. | |
dc.contributor.author | Lim, S.M. | |
dc.contributor.author | Goulburn, A.L. | |
dc.contributor.author | Micallef, S.J. | |
dc.contributor.author | Labonne, T. | |
dc.contributor.author | Kicic, Anthony | |
dc.contributor.author | Ling, K.M. | |
dc.contributor.author | Stick, S.M. | |
dc.contributor.author | Ng, E.S. | |
dc.contributor.author | Trounson, A. | |
dc.contributor.author | Giudice, A. | |
dc.contributor.author | Elefanty, A.G. | |
dc.contributor.author | Stanley, E.G. | |
dc.date.accessioned | 2019-11-10T02:17:32Z | |
dc.date.available | 2019-11-10T02:17:32Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Jenny, R.A. and Hirst, C. and Lim, S.M. and Goulburn, A.L. and Micallef, S.J. and Labonne, T. and Kicic, A. et al. 2015. Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus. Stem Cells Translational Medicine. 4 (6): pp. 603-614. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/76816 | |
dc.identifier.doi | 10.5966/sctm.2014-0274 | |
dc.description.abstract |
© AlphaMed Press 2015. Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter humanembryonic stem cell line, wede-veloped a serum-free protocol for the generation of NKX2.1 < sup > + < /sup > endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. Gene profiling experiments indicated that day 10 NKX2.1 < sup > + < /sup > endo-derm expressed markers indicative of early foregut but lacked genes associated with later stages of respiratory epithelial cell differentiation. Nevertheless, NKX2.1 < sup > + < /sup > endoderm supported the infection and replication of the common respiratory pathogen human rhinovirus HRV1b. Moreover, NKX2.1 < sup > + < /sup > endoderm upregulated expression of IL-6, IL-8, and IL-1B in response to infection, a characteristic of human airway epithelial cells. Our experiments provide proof of principle for the use of PSC-derived respiratory epithelial cells in the study of cell-virus interactions. | |
dc.language | English | |
dc.publisher | ALPHAMED PRESS | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Cell & Tissue Engineering | |
dc.subject | Cell Biology | |
dc.subject | Human embryonic stem cells | |
dc.subject | NKX2.1 | |
dc.subject | Respiratory endoderm | |
dc.subject | Lung | |
dc.subject | HRV | |
dc.subject | Differentiation | |
dc.subject | Rhinovirus | |
dc.subject | GFP | |
dc.subject | ANTERIOR FOREGUT ENDODERM | |
dc.subject | TRANSCRIPTION FACTOR-I | |
dc.subject | PDGF RECEPTOR-ALPHA | |
dc.subject | DIRECTED DIFFERENTIATION | |
dc.subject | DEFINITIVE ENDODERM | |
dc.subject | LUNG MORPHOGENESIS | |
dc.subject | PRIMITIVE STREAK | |
dc.subject | AIRWAY EPITHELIA | |
dc.subject | CYSTIC-FIBROSIS | |
dc.subject | VITRO MODEL | |
dc.title | Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus | |
dc.type | Journal Article | |
dcterms.source.volume | 4 | |
dcterms.source.number | 6 | |
dcterms.source.startPage | 603 | |
dcterms.source.endPage | 614 | |
dcterms.source.issn | 2157-6564 | |
dcterms.source.title | Stem Cells Translational Medicine | |
dc.date.updated | 2019-11-10T02:17:26Z | |
curtin.department | School of Public Health | |
curtin.accessStatus | Fulltext not available | |
curtin.faculty | Faculty of Health Sciences | |
curtin.contributor.orcid | Kicic, Anthony [0000-0002-0008-9733] | |
dcterms.source.eissn | 2157-6580 | |
curtin.contributor.scopusauthorid | Kicic, Anthony [6507472922] |