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dc.contributor.authorJenny, R.A.
dc.contributor.authorHirst, C.
dc.contributor.authorLim, S.M.
dc.contributor.authorGoulburn, A.L.
dc.contributor.authorMicallef, S.J.
dc.contributor.authorLabonne, T.
dc.contributor.authorKicic, Anthony
dc.contributor.authorLing, K.M.
dc.contributor.authorStick, S.M.
dc.contributor.authorNg, E.S.
dc.contributor.authorTrounson, A.
dc.contributor.authorGiudice, A.
dc.contributor.authorElefanty, A.G.
dc.contributor.authorStanley, E.G.
dc.date.accessioned2019-11-10T02:17:32Z
dc.date.available2019-11-10T02:17:32Z
dc.date.issued2015
dc.identifier.citationJenny, R.A. and Hirst, C. and Lim, S.M. and Goulburn, A.L. and Micallef, S.J. and Labonne, T. and Kicic, A. et al. 2015. Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus. Stem Cells Translational Medicine. 4 (6): pp. 603-614.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/76816
dc.identifier.doi10.5966/sctm.2014-0274
dc.description.abstract

© AlphaMed Press 2015. Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter humanembryonic stem cell line, wede-veloped a serum-free protocol for the generation of NKX2.1 < sup > + < /sup > endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. Gene profiling experiments indicated that day 10 NKX2.1 < sup > + < /sup > endo-derm expressed markers indicative of early foregut but lacked genes associated with later stages of respiratory epithelial cell differentiation. Nevertheless, NKX2.1 < sup > + < /sup > endoderm supported the infection and replication of the common respiratory pathogen human rhinovirus HRV1b. Moreover, NKX2.1 < sup > + < /sup > endoderm upregulated expression of IL-6, IL-8, and IL-1B in response to infection, a characteristic of human airway epithelial cells. Our experiments provide proof of principle for the use of PSC-derived respiratory epithelial cells in the study of cell-virus interactions.

dc.languageEnglish
dc.publisherALPHAMED PRESS
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectCell & Tissue Engineering
dc.subjectCell Biology
dc.subjectHuman embryonic stem cells
dc.subjectNKX2.1
dc.subjectRespiratory endoderm
dc.subjectLung
dc.subjectHRV
dc.subjectDifferentiation
dc.subjectRhinovirus
dc.subjectGFP
dc.subjectANTERIOR FOREGUT ENDODERM
dc.subjectTRANSCRIPTION FACTOR-I
dc.subjectPDGF RECEPTOR-ALPHA
dc.subjectDIRECTED DIFFERENTIATION
dc.subjectDEFINITIVE ENDODERM
dc.subjectLUNG MORPHOGENESIS
dc.subjectPRIMITIVE STREAK
dc.subjectAIRWAY EPITHELIA
dc.subjectCYSTIC-FIBROSIS
dc.subjectVITRO MODEL
dc.titleProductive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus
dc.typeJournal Article
dcterms.source.volume4
dcterms.source.number6
dcterms.source.startPage603
dcterms.source.endPage614
dcterms.source.issn2157-6564
dcterms.source.titleStem Cells Translational Medicine
dc.date.updated2019-11-10T02:17:26Z
curtin.departmentSchool of Public Health
curtin.accessStatusFulltext not available
curtin.facultyFaculty of Health Sciences
curtin.contributor.orcidKicic, Anthony [0000-0002-0008-9733]
dcterms.source.eissn2157-6580
curtin.contributor.scopusauthoridKicic, Anthony [6507472922]


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