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    Characterization of side population cells from human airway epithelium

    Access Status
    Fulltext not available
    Authors
    Hackett, T.L.
    Shaheen, F.
    Johnson, A.
    Wadsworth, S.
    Pechkovsky, D.V.
    Jacoby, D.B.
    Kicic, Anthony
    Stick, S.M.
    Knight, D.A.
    Date
    2008
    Type
    Journal Article
    
    Metadata
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    Citation
    Hackett, T.L. and Shaheen, F. and Johnson, A. and Wadsworth, S. and Pechkovsky, D.V. and Jacoby, D.B. and Kicic, A. et al. 2008. Characterization of side population cells from human airway epithelium. Stem Cells. 26 (10): pp. 2576-2585.
    Source Title
    Stem Cells
    DOI
    10.1634/stemcells.2008-0171
    ISSN
    1066-5099
    Faculty
    Faculty of Health Sciences
    School
    School of Public Health
    URI
    http://hdl.handle.net/20.500.11937/76825
    Collection
    • Curtin Research Publications
    Abstract

    The airway epithelium is the first line of contact with the inhaled external environment and is continuously exposed to and injured by pollutants, allergens, and viruses. However, little is known about epithelial repair and in particular the identity and role of tissue resident stem/progenitor cells that may contribute to epithelial regeneration. The aims of the present study were to identify, isolate, and characterize side population (SP) cells in human tracheobronchial epithelium. Epithelial cells were obtained from seven nontransplantable healthy lungs and four asthmatic lungs by pronase digestion. SP cells were identified by verapamil-sensitive efflux of the DNA-binding dye Hoechst 33342. Using flow cytometry, CD45- SP, CD45+ SP, and non-SP cells were isolated and sorted. CD45- SP cells made up 0.12% ± 0.01% of the total epithelial cell population in normal airway but 4.1% ± 0.06% of the epithelium in asthmatic airways. All CD45 - SP cells showed positive staining for epithelial-specific markers cytokeratin-5, E-cadherin, ZO-1, and p63. CD45- SP cells exhibited stable telomere length and increased colony-forming and proliferative potential, undergoing population expansion for at least 16 consecutive passages. In contrast with non-SP cells, fewer than 100 CD45- SP cells were able to generate a multilayered and differentiated epithelium in air-liquid interface culture. SP cells are present in human tracheobronchial epithelium, exhibit both short- and long-term proliferative potential, and are capable of generation of differentiated epithelium in vitro. The number of SP cells is significantly greater in asthmatic airways, providing evidence of dysregulated resident SP cells in the asthmatic epithelium. ©AlphaMed Press.

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