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dc.contributor.authorHackett, T.L.
dc.contributor.authorShaheen, F.
dc.contributor.authorJohnson, A.
dc.contributor.authorWadsworth, S.
dc.contributor.authorPechkovsky, D.V.
dc.contributor.authorJacoby, D.B.
dc.contributor.authorKicic, Anthony
dc.contributor.authorStick, S.M.
dc.contributor.authorKnight, D.A.
dc.date.accessioned2019-11-10T02:32:13Z
dc.date.available2019-11-10T02:32:13Z
dc.date.issued2008
dc.identifier.citationHackett, T.L. and Shaheen, F. and Johnson, A. and Wadsworth, S. and Pechkovsky, D.V. and Jacoby, D.B. and Kicic, A. et al. 2008. Characterization of side population cells from human airway epithelium. Stem Cells. 26 (10): pp. 2576-2585.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/76825
dc.identifier.doi10.1634/stemcells.2008-0171
dc.description.abstract

The airway epithelium is the first line of contact with the inhaled external environment and is continuously exposed to and injured by pollutants, allergens, and viruses. However, little is known about epithelial repair and in particular the identity and role of tissue resident stem/progenitor cells that may contribute to epithelial regeneration. The aims of the present study were to identify, isolate, and characterize side population (SP) cells in human tracheobronchial epithelium. Epithelial cells were obtained from seven nontransplantable healthy lungs and four asthmatic lungs by pronase digestion. SP cells were identified by verapamil-sensitive efflux of the DNA-binding dye Hoechst 33342. Using flow cytometry, CD45- SP, CD45+ SP, and non-SP cells were isolated and sorted. CD45- SP cells made up 0.12% ± 0.01% of the total epithelial cell population in normal airway but 4.1% ± 0.06% of the epithelium in asthmatic airways. All CD45 - SP cells showed positive staining for epithelial-specific markers cytokeratin-5, E-cadherin, ZO-1, and p63. CD45- SP cells exhibited stable telomere length and increased colony-forming and proliferative potential, undergoing population expansion for at least 16 consecutive passages. In contrast with non-SP cells, fewer than 100 CD45- SP cells were able to generate a multilayered and differentiated epithelium in air-liquid interface culture. SP cells are present in human tracheobronchial epithelium, exhibit both short- and long-term proliferative potential, and are capable of generation of differentiated epithelium in vitro. The number of SP cells is significantly greater in asthmatic airways, providing evidence of dysregulated resident SP cells in the asthmatic epithelium. ©AlphaMed Press.

dc.languageEnglish
dc.publisherWILEY
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectCell & Tissue Engineering
dc.subjectBiotechnology & Applied Microbiology
dc.subjectOncology
dc.subjectCell Biology
dc.subjectHematology
dc.subjectEpithelium
dc.subjectTissue-specific stem cell
dc.subjectHuman
dc.subjectAsthma
dc.subjectSTEM-CELLS
dc.subjectFUNCTIONAL-PROPERTIES
dc.subjectPROGENITOR CELLS
dc.subjectIN-VITRO
dc.subjectEXPRESSION
dc.subjectTELOMERASE
dc.subjectADULT
dc.subjectLUNG
dc.subjectPROLIFERATION
dc.subjectTRANSPORTER
dc.titleCharacterization of side population cells from human airway epithelium
dc.typeJournal Article
dcterms.source.volume26
dcterms.source.number10
dcterms.source.startPage2576
dcterms.source.endPage2585
dcterms.source.issn1066-5099
dcterms.source.titleStem Cells
dc.date.updated2019-11-10T02:32:07Z
curtin.departmentSchool of Public Health
curtin.accessStatusFulltext not available
curtin.facultyFaculty of Health Sciences
curtin.contributor.orcidKicic, Anthony [0000-0002-0008-9733]
dcterms.source.eissn1549-4918
curtin.contributor.scopusauthoridKicic, Anthony [6507472922]


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