Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene
MetadataShow full item record
The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism. © 2011, American Society for Microbiology. All Rights Reserved.
Showing items related by title, author, creator and subject.
The molecular genetics of human complement C4: implications for mapping MHC disease susceptibility genesPuschendorf, Mareike (2003)The Major Histocompatibility Complex (MHC) is a gene-dense region located on the short arm of chromosome 6 (6p21.31). This region contains the highly polymorphic HLA genes as well as many other genes with immunological ...
Analysis of candidate genes within the 3p14-p22 region of the human genome for association with bone mineral density phenotypesMullin, Benjamin H (2011)Previous studies have identified the 3p14-p22 chromosomal region as a quantitative trait locus for bone mineral density (BMD). The overall aim of this thesis is to identify the gene or genes from this region that are ...
Secondary students' understanding of the gene concept : an analysis of conceptual change from multiple perspectives.Venville, Grady J. (1997)A journey into the past century of genetics history reveals transformations of the concept of the gene through notions of discrete units that obeyed Mendelian laws to the modem bewildering gene concept. We can no longer ...