Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene
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Authors
Christophersen, Claus
Morrison, M.
Conlon, M.A.
Date
2011Type
Journal Article
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Christophersen, C.T. and Morrison, M. and Conlon, M.A. 2011. Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene. Applied and Environmental Microbiology. 77 (10): pp. 3544-3546.
Source Title
Applied and Environmental Microbiology
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Faculty
Faculty of Science and Engineering
School
School of Molecular and Life Sciences (MLS)
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Abstract
The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism. © 2011, American Society for Microbiology. All Rights Reserved.