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dc.contributor.authorChristophersen, Claus
dc.contributor.authorMorrison, M.
dc.contributor.authorConlon, M.A.
dc.date.accessioned2020-05-14T04:44:02Z
dc.date.available2020-05-14T04:44:02Z
dc.date.issued2011
dc.identifier.citationChristophersen, C.T. and Morrison, M. and Conlon, M.A. 2011. Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene. Applied and Environmental Microbiology. 77 (10): pp. 3544-3546.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/79137
dc.identifier.doi10.1128/AEM.02851-10
dc.description.abstract

The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism. © 2011, American Society for Microbiology. All Rights Reserved.

dc.languageEnglish
dc.publisherAMER SOC MICROBIOLOGY
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiotechnology & Applied Microbiology
dc.subjectMicrobiology
dc.subjectPOLYMERASE-CHAIN-REACTION
dc.subjectREAL-TIME PCR
dc.subjectULCERATIVE-COLITIS
dc.subjectGASTROINTESTINAL-TRACT
dc.subjectHYDROGEN-SULFIDE
dc.subjectCOLORECTAL-CANCER
dc.subjectHEALTHY
dc.subjectQUANTIFICATION
dc.subjectIDENTIFICATION
dc.subjectPOPULATIONS
dc.titleOverestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene
dc.typeJournal Article
dcterms.source.volume77
dcterms.source.number10
dcterms.source.startPage3544
dcterms.source.endPage3546
dcterms.source.issn0099-2240
dcterms.source.titleApplied and Environmental Microbiology
dc.date.updated2020-05-14T04:44:01Z
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusFulltext not available
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidChristophersen, Claus [0000-0003-1591-5871]
dcterms.source.eissn1098-5336
curtin.contributor.scopusauthoridChristophersen, Claus [7006206487]


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