Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies
Access Status
Fulltext not available
Authors
Sheng, H.Z.
Martenson, R.E.
Grgacic, E.V.
Dowse, Carol
Carnegie, R.L.
Bernard, C.C.A.
Date
1988Type
Journal Article
Metadata
Show full item recordCitation
Sheng, H.Z. and Martenson, R.E. and Grgacic, E.V. and Dowse, C.A. and Carnegie, R.L. and Bernard, C.C.A. 1988. Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies. Journal of Neuroimmunology. 17 (3): pp. 183-192.
Source Title
Journal of Neuroimmunology
ISSN
Faculty
Faculty of Business and Law
Collection
Abstract
A rapid and sensitive method for the identification of antigenic determinants recognised by monoclonal and polyclonal antibodies directed against myelin basic protein (MBP) is described. By electroimmunoblotting a series of overlapping peptides covering the entire MBP molecule with monoclonal anti-MBP antibodies, the binding pattern of immunoreactive peptides can be rapidly determined and the reactive antigenic determinant identified. This procedure, which can be performed with both native and synthetic peptides, can also with appropriate modification, be applied to the analysis of naturally occuring or experimentally induced polyclonal anti-MBP autoantibodies. © 1988.