Endotoxin removal and plasmid DNA recovery in various metal ion solutions
Abstract
In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimum combination of high endotoxin removal efficiency (~91%) as well as high plasmid recovery (~100%) compared to other metal ions tested.
Citation
Ongkudon, C. and Danquah, M. 2011. Endotoxin removal and plasmid DNA recovery in various metal ion solutions. Separation Science and Technology. 46 (8): pp. 1280-1282.
Source Title
Separation Science and Technology
Additional URLs
ISSN
School
Curtin Sarawak
Collection
Related items
Showing items related by title, author, creator and subject.
-
Terdkiatburana, Thanet (2007)Humic substances are macromolecules that naturally occur in all environments in which vegetation matter are present. In general, humic acid is part of humic substances which form the major fraction of the dissolved organic ...
-
Rajmohan, N.; Prathapar, S.; Jayaprakash, M.; Ramasamy, Nagarajan (2014)The accumulation of heavy metals in soil and water is a serious concern due to their persistence and toxicity. This study investigated the vertical distribution of heavy metals, possible sources and their relation with ...
-
Sen, Tushar; Mei, C. (2012)In this work the adsorptive properties of aluminium oxide in the removal of zinc (Zn2+) from aqueous solution have been studied by laboratory batch adsorption kinetic and equilibrium experiments.The results show that the ...