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    Endotoxin removal and plasmid DNA recovery in various metal ion solutions

    Access Status
    Fulltext not available
    Authors
    Ongkudon, C.
    Danquah, Michael
    Date
    2011
    Type
    Journal Article
    
    Metadata
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    Citation
    Ongkudon, C. and Danquah, M. 2011. Endotoxin removal and plasmid DNA recovery in various metal ion solutions. Separation Science and Technology. 46 (8): pp. 1280-1282.
    Source Title
    Separation Science and Technology
    DOI
    10.1080/01496395.2011.557027
    Additional URLs
    http://www.tandfonline.com/doi/full/10.1080/01496395.2011.557027
    ISSN
    0149-6395
    School
    Curtin Sarawak
    URI
    http://hdl.handle.net/20.500.11937/8774
    Collection
    • Curtin Research Publications
    Abstract

    In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimum combination of high endotoxin removal efficiency (~91%) as well as high plasmid recovery (~100%) compared to other metal ions tested.

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