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    Immunohistochemical evidence of P2X7R, P2X4R and CaMKK2 in pyramidal neurons of frontal cortex does not align with Alzheimer's disease

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    Authors
    Gaff, Jessica
    Jackaman, Connie
    Papadimitriou, J.
    Waters, Shelley
    McLean, C.
    Price, Patricia
    Date
    2021
    Type
    Journal Article
    
    Metadata
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    Citation
    Gaff, J. and Jackaman, C. and Papadimitriou, J. and Waters, S. and McLean, C. and Price, P. 2021. Immunohistochemical evidence of P2X7R, P2X4R and CaMKK2 in pyramidal neurons of frontal cortex does not align with Alzheimer's disease. Experimental and Molecular Pathology. 120: ARTN 104636.
    Source Title
    Experimental and Molecular Pathology
    DOI
    10.1016/j.yexmp.2021.104636
    ISSN
    0014-4800
    Faculty
    Faculty of Health Sciences
    School
    Curtin Medical School
    URI
    http://hdl.handle.net/20.500.11937/89469
    Collection
    • Curtin Research Publications
    Abstract

    Alzheimer's disease (AD) is an incurable neurodegenerative condition resulting in progressive cognitive decline. Pathological features include Aβ plaques, neurofibrillary tangles, neuroinflammation and neuronal death. Purinergic receptors 7 and 4 (P2X7R and P2X4R) and calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) are implicated in neuronal death. We used immunohistochemistry to investigate the distribution of these proteins in neurones from frontal cortex of donors (n = 3/group; aged 79–83 years) who died with and without AD. Neurones were identified morphologically and immunoperoxidase staining was achieved using commercial antibodies. Immunoreactive neurones were counted for each protein by 2–3 raters blinded to the diagnoses. We observed no differences in percentages of P2X7R, P2X4R or CaMKK2 positive neurones (p = 0.2–0.99), but sections from individuals with AD had marginally fewer neurones (p = 0.10). Hence P2X7R, P2X4R or CaMKK2 appear to be expressed in neurones from older donors, but expression does not associate with AD.

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