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dc.contributor.authorZannah, Shaheda
dc.contributor.authorArrigan, Damien
dc.date.accessioned2022-11-03T13:14:46Z
dc.date.available2022-11-03T13:14:46Z
dc.date.issued2021
dc.identifier.citationZannah, S. and Arrigan, D. 2021. Electrochemistry of catalase at a liquid. Bioelectrochemistry. 138: ARTN 107694.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/89594
dc.identifier.doi10.1016/j.bioelechem.2020.107694
dc.description.abstract

The electrochemistry of catalase (CAT) was investigated at the interface between two immiscible electrolyte solutions (ITIES) as a step towards its detection. Electrochemistry at the ITIES offers advantages such as the non-redox detection of biomolecules. The electrochemical behaviour of CAT at the ITIES, in a micro-interface array format, displayed a distinct cyclic voltammogram when the aqueous phase pH was lower than the isoelectric point (pI) of CAT. No voltammetric response was observed when the aqueous phase pH > pI of CAT, indicating that neutral or negatively charged CAT has no capability to facilitate anion transfer from the organic phase. Adsorptive stripping voltammetry (AdSV) was assessed for detection of low concentrations at the µITIES array. Application of a positive preconcentration potential for a fixed time enabled interfacial accumulation of CAT as a complex; subsequently, a voltammetric scan to lower potentials desorbed the complex, providing the electroanalytical signal. Assessment of sample matrix effects by examining the electrochemistry of CAT in artificial serum indicated that detection in pH-adjusted samples is feasible. Together, these results demonstrate that CAT is electroactive at the liquid–liquid interface and this may be useful as a strategy to detect and characterize the enzyme in a label-free manner.

dc.languageEnglish
dc.publisherELSEVIER SCIENCE SA
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysical Sciences
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiology
dc.subjectBiophysics
dc.subjectElectrochemistry
dc.subjectLife Sciences & Biomedicine - Other Topics
dc.subjectCatalase
dc.subjectMicro-interface
dc.subjectVoltammetry
dc.subjectAdsorption
dc.subjectITIES
dc.subjectION-TRANSFER VOLTAMMETRY
dc.subjectEGG-WHITE-LYSOZYME
dc.subjectBEHAVIOR
dc.subjectHEMOGLOBIN
dc.subjectEXPRESSION
dc.subjectBIOMARKERS
dc.subjectPROTEINS
dc.subjectINSULIN
dc.subjectBINDING
dc.titleElectrochemistry of catalase at a liquid
dc.typeJournal Article
dcterms.source.volume138
dcterms.source.issn1567-5394
dcterms.source.titleBioelectrochemistry
dc.date.updated2022-11-03T13:14:45Z
curtin.accessStatusOpen access
curtin.contributor.orcidArrigan, Damien [0000-0002-1053-1273]
curtin.contributor.researcheridArrigan, Damien [A-7440-2010]
curtin.identifier.article-numberARTN 107694
dcterms.source.eissn1878-562X
curtin.contributor.scopusauthoridArrigan, Damien [7004238830]


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