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dc.contributor.authorBelotti, Mattia
dc.contributor.authorEl-Tahawy, M.M.T.
dc.contributor.authorYu, L.J.
dc.contributor.authorRussell, I.C.
dc.contributor.authorDarwish, Nadim
dc.contributor.authorCoote, M.L.
dc.contributor.authorGaravelli, M.
dc.contributor.authorCiampi, Simone
dc.date.accessioned2023-02-14T08:46:48Z
dc.date.available2023-02-14T08:46:48Z
dc.date.issued2022
dc.identifier.citationBelotti, M. and El-Tahawy, M.M.T. and Yu, L.J. and Russell, I.C. and Darwish, N. and Coote, M.L. and Garavelli, M. et al. 2022. Luciferase-free Luciferin Electrochemiluminescence. Angewandte Chemie - International Edition. 61 (46): e202209670.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/90478
dc.identifier.doi10.1002/anie.202209670
dc.description.abstract

Luciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst—luciferase. We have gained experimental and computational insights on the mechanism of the enzyme-free luciferin electrochemiluminescence, demonstrated its spectral tuning from green to red by means of electrolyte engineering, proven that the colour change does not require, as still debated, a keto/enol isomerization of the light emitter, and gained evidence of the electrostatic-assisted stabilization of the charge-transfer excited state by double layer electric fields. Luciferin's electrochemiluminescence, as well as the in situ generation of fluorescent oxyluciferin, are applied towards an optical measurement of diffusion coefficients.

dc.languageEnglish
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.sponsoredbyhttp://purl.org/au-research/grants/arc/DP190100735
dc.relation.sponsoredbyhttp://purl.org/au-research/grants/arc/FT190100148
dc.relation.sponsoredbyhttp://purl.org/au-research/grants/arc/FL170100041
dc.relation.sponsoredbyhttp://purl.org/au-research/grants/arc/CE140100012
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectScience & Technology
dc.subjectPhysical Sciences
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry
dc.subjectElectrochemiluminescence
dc.subjectElectrochemistry
dc.subjectElectrostatic Interactions
dc.subjectProfluorescence
dc.subjectQuantum Chemistry
dc.subjectELECTRICAL DOUBLE-LAYER
dc.subjectFIREFLY LUCIFERIN
dc.subjectSPECTRAL DISTRIBUTION
dc.subjectBIOLUMINESCENCE
dc.subjectCHEMILUMINESCENCE
dc.subjectMECHANISM
dc.subjectMODULATION
dc.subjectLIGHT
dc.subjectLUMINESCENCE
dc.subjectOXIDATION
dc.titleLuciferase-free Luciferin Electrochemiluminescence
dc.typeJournal Article
dcterms.source.volume61
dcterms.source.number46
dcterms.source.issn1433-7851
dcterms.source.titleAngewandte Chemie - International Edition
dc.date.updated2023-02-14T08:46:47Z
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidDarwish, Nadim [0000-0002-6565-1723]
curtin.contributor.orcidCiampi, Simone [0000-0002-8272-8454]
curtin.contributor.orcidBelotti, Mattia [0000-0002-7382-337X]
curtin.contributor.researcheridCiampi, Simone [D-9129-2014]
curtin.identifier.article-numbere202209670
dcterms.source.eissn1521-3773
curtin.contributor.scopusauthoridDarwish, Nadim [14031207900]
curtin.contributor.scopusauthoridCiampi, Simone [21733701500]


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