Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    X-ray fluorescence microscopy methods for biological tissues

    Access Status
    Open access via publisher
    Authors
    Pushie, M.J.
    Sylvain, N.J.
    Hou, H.
    Hackett, Mark
    Kelly, M.E.
    Webb, S.M.
    Date
    2022
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Pushie, M.J. and Sylvain, N.J. and Hou, H. and Hackett, M.J. and Kelly, M.E. and Webb, S.M. 2022. X-ray fluorescence microscopy methods for biological tissues. Metallomics. 14 (6): ARTN mfac032.
    Source Title
    Metallomics
    DOI
    10.1093/mtomcs/mfac032
    ISSN
    1756-5901
    Faculty
    Faculty of Science and Engineering
    School
    School of Molecular and Life Sciences (MLS)
    URI
    http://hdl.handle.net/20.500.11937/92982
    Collection
    • Curtin Research Publications
    Abstract

    Synchrotron-based X-ray fluorescence microscopy is a flexible tool for identifying the distribution of trace elements in biological specimens across a broad range of sample sizes. The technique is not particularly limited by sample type and can be performed on ancient fossils, fixed or fresh tissue specimens, and in some cases even live tissue and live cells can be studied. The technique can also be expanded to provide chemical specificity to elemental maps, either at individual points of interest in a map or across a large field of view. While virtually any sample type can be characterized with X-ray fluorescence microscopy, common biological sample preparation methods ( often borrowed from other fields, such as histology ) can lead to unforeseen pitfalls, resulting in altered element distributions and concentrations. A general overview of sample preparation and data-acquisition methods for X-ray fluorescence microscopy is presented, along with outlining the general approach for applying this technique to a new field of investigation for prospective new users. Considerations for improving data acquisition and quality are reviewed as well as the effects of sample prepa- ration, with a particular focus on soft tissues. The effects of common sample pretreatment steps as well as the underlying factors that govern which, and to what extent, specific elements are likely to be altered are reviewed along with common artifacts observed in X-ray fluorescence microscopy data.

    Related items

    Showing items related by title, author, creator and subject.

    • Elemental characterisation of the pyramidal neuron layer within the rat and mouse hippocampus.
      Hackett, Mark; Ashley Hollings; Caine, S.; Bewer, B.; Alaverdashvili, M.; Takechi, Ryu; Mamo, John; Jones, M.; de Jonge, M.; Paterson, P.; Pickering, I.; George, G. (2018)
      A unique combination of sensitivity, resolution, and penetration make X-ray fluorescence imaging (XFI) ideally suited to investigate trace elemental distributions in the biological context. XFI has gained widespread use ...
    • Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging.
      Wedding, J.; Harris, H.; Bader, C.; Plush, S.; Mak, R.; Massi, M.; Brooks, D.; Lai, B.; Vogt, S.; Werrett, M.; Simpson, Peter; Skelton, B.; Stagni, S. (2016)
      Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = ...
    • Revealing the Penumbra through Imaging Elemental Markers of Cellular Metabolism in an Ischemic Stroke Model
      Pushie, M.; Crawford, A.; Sylvain, N.; Hou, H.; Hackett, Mark; George, G.; Kelly, M. (2018)
      Stroke exacts a heavy financial and economic burden, is a leading cause of death, and is the leading cause of long-term disability in those who survive. The penumbra surrounds the ischemic core of the stroke lesion and ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.