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dc.contributor.authorMorikawa, Shota
dc.contributor.authorVerdonk, Callum
dc.contributor.authorJohn, Evan
dc.contributor.authorLenzo, Leon
dc.contributor.authorSbaraini, Nicolau
dc.contributor.authorTuro, Chala
dc.contributor.authorLi, Hang
dc.contributor.authorJiang, David
dc.contributor.authorChooi, Y.H.
dc.contributor.authorTan, Kar-Chun
dc.date.accessioned2024-10-07T05:20:50Z
dc.date.available2024-10-07T05:20:50Z
dc.date.issued2024
dc.identifier.citationMorikawa, S. and Verdonk, C. and John, E. and Lenzo, L. and Sbaraini, N. and Turo, C. and Li, H. et al. 2024. The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum. BMC Microbiology. 24 (1): 299.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/96023
dc.identifier.doi10.1186/s12866-024-03454-7
dc.description.abstract

The fungus Parastagonospora nodorum causes septoria nodorum blotch on wheat. The role of the fungal Velvet-family transcription factor VeA in P. nodorum development and virulence was investigated here. Deletion of the P. nodorum VeA ortholog, PnVeA, resulted in growth abnormalities including pigmentation, abolished asexual sporulation and highly reduced virulence on wheat. Comparative RNA-Seq and RT-PCR analyses revealed that the deletion of PnVeA also decoupled the expression of major necrotrophic effector genes. In addition, the deletion of PnVeA resulted in an up-regulation of four predicted secondary metabolite (SM) gene clusters. Using liquid-chromatography mass-spectrometry, it was observed that one of the SM gene clusters led to an accumulation of the mycotoxin alternariol. PnVeA is essential for asexual sporulation, full virulence, secondary metabolism and necrotrophic effector regulation.

dc.languageeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParastagonospora nodorum
dc.subjectNecrotrophic effector
dc.subjectSecondary metabolite
dc.subjectVelvet A
dc.subjectWheat
dc.subjectAscomycota
dc.subjectFungal Proteins
dc.subjectGene Expression Regulation, Fungal
dc.subjectLactones
dc.subjectMultigene Family
dc.subjectMycotoxins
dc.subjectPlant Diseases
dc.subjectSecondary Metabolism
dc.subjectSpores, Fungal
dc.subjectTranscription Factors
dc.subjectTriticum
dc.subjectVirulence
dc.subjectAscomycota
dc.subjectSpores, Fungal
dc.subjectTriticum
dc.subjectLactones
dc.subjectFungal Proteins
dc.subjectTranscription Factors
dc.subjectMycotoxins
dc.subjectVirulence
dc.subjectPlant Diseases
dc.subjectGene Expression Regulation, Fungal
dc.subjectMultigene Family
dc.subjectSecondary Metabolism
dc.titleThe Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum
dc.typeJournal Article
dcterms.source.volume24
dcterms.source.number1
dcterms.source.issn1471-2180
dcterms.source.titleBMC Microbiology
dc.date.updated2024-10-07T05:20:43Z
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidTuro, Chala [0000-0001-7247-5900]
curtin.contributor.orcidTan, Kar-Chun [0000-0001-6094-823X]
curtin.identifier.article-number299
dcterms.source.eissn1471-2180
curtin.contributor.scopusauthoridTuro, Chala [57200645040]
curtin.contributor.scopusauthoridTan, Kar-Chun [56231324800] [7403999337]
curtin.repositoryagreementV3


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