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dc.contributor.authorDiBattista, J.D.
dc.contributor.authorLiu, S.Y.V.
dc.contributor.authorDe Brauwer, M.
dc.contributor.authorWilkinson, S.P.
dc.contributor.authorWest, K.
dc.contributor.authorKoziol, A.
dc.contributor.authorBunce, Michael
dc.date.accessioned2024-11-06T09:58:52Z
dc.date.available2024-11-06T09:58:52Z
dc.date.issued2023
dc.identifier.citationDiBattista, J.D. and Liu, S.Y.V. and De Brauwer, M. and Wilkinson, S.P. and West, K. and Koziol, A. and Bunce, M. 2023. Gut content metabarcoding of specialized feeders is not a replacement for environmental DNA assays of seawater in reef environments. PeerJ. 11: pp. e16075-.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/96303
dc.identifier.doi10.7717/peerj.16075
dc.description.abstract

In tropical marine ecosystems, the coral-based diet of benthic-feeding reef fishes provides a window into the composition and health of coral reefs. In this study, for the first time, we compare multi-assay metabarcoding sequences of environmental DNA (eDNA) isolated from seawater and partially digested gut items from an obligate corallivore butterflyfish (Chaetodon lunulatus) resident to coral reef sites in the South China Sea. We specifically tested the proportional and statistical overlap of the different approaches (seawater vs gut content metabarcoding) in characterizing eukaryotic community composition on coral reefs. Based on 18S and ITS2 sequence data, which differed in their taxonomic sensitivity, we found that gut content detections were only partially representative of the eukaryotic communities detected in the seawater based on low levels of taxonomic overlap (3 to 21%) and significant differences between the sampling approaches. Overall, our results indicate that dietary metabarcoding of specialized feeders can be complimentary to, but is no replacement for, more comprehensive environmental DNA assays of reef environments that might include the processing of different substrates (seawater, sediment, plankton) or traditional observational surveys. These molecular assays, in tandem, might be best suited to highly productive but cryptic oceanic environments (kelp forests, seagrass meadows) that contain an abundance of organisms that are often small, epiphytic, symbiotic, or cryptic.

dc.languageeng
dc.subjectAdaptation
dc.subjectChaetodon lunulatus
dc.subjectCoral reefs
dc.subjectEnvironmental DNA
dc.subjectReef fish
dc.subjectSouth China Sea
dc.subjectAnimals
dc.subjectEcosystem
dc.subjectDNA, Environmental
dc.subjectCoral Reefs
dc.subjectAnthozoa
dc.subjectSeawater
dc.subjectAnimals
dc.subjectAnthozoa
dc.subjectEcosystem
dc.subjectSeawater
dc.subjectCoral Reefs
dc.subjectDNA, Environmental
dc.titleGut content metabarcoding of specialized feeders is not a replacement for environmental DNA assays of seawater in reef environments
dc.typeJournal Article
dcterms.source.volume11
dcterms.source.startPagee16075
dcterms.source.issn2167-8359
dcterms.source.titlePeerJ
dc.date.updated2024-11-06T09:58:43Z
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusIn process
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidBunce, Michael [0000-0002-0302-4206]
dcterms.source.eissn2167-8359
curtin.contributor.scopusauthoridBunce, Michael [55160482300]
curtin.repositoryagreementV3


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