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    Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response

    Access Status
    Fulltext not available
    Authors
    Nongonierma, Alice
    Gaudel, Celine
    Murray, Brian
    Flynn, Sarah
    Kelly, Phillip
    Newsholme, Philip
    FitzGerald, Richard
    Date
    2013
    Type
    Journal Article
    
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    Citation
    Nongonierma, Alice and Gaudel, Celine and Murray, Brian and Flynn, Sarah and Kelly, Phillip and Newsholme, Philip and FitzGerald, Richard. 2013. Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response. International Dairy Journal. 32: pp. 163-168.
    Source Title
    International Dairy Journal
    DOI
    10.1016/j.idairyj.2013.05.014
    ISSN
    0958-6946
    URI
    http://hdl.handle.net/20.500.11937/10693
    Collection
    • Curtin Research Publications
    Abstract

    The influence of different substrates and the effect of pH regulation during enzymatic hydrolysis of whey protein on glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells were studied. No significant differences (P ≥ 0.05) were detected in terms of glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells exposed to whey protein hydrolysates (WPH1 and WPH2) obtained with two different whey protein substrates. The whey protein hydrolysate (WPH3) obtained without pH regulation during hydrolysis, had a significantly lower insulinotropic potential in BRIN-BD11 cells than the WPH1 hydrolysate that was manufactured with pH regulation. Fractionation of WPH1 was carried out to enrich bioactive peptides. Comparing the different fractionation techniques studied (solid-phase extraction and semi-preparative reverse phase-high performance liquid chromatography), the most potent insulinotropic fraction was enriched in free amino acids and contained relatively hydrophilic peptides. This indicates that amino acids and hydrophilic peptides may be involved in the insulinotropic effect of WPH1.

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