Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells

    Access Status
    Fulltext not available
    Authors
    Tapadia, M.
    Carlessi, Rodrigo
    Johnson, Stuart
    Utikar, Ranjeet
    Newsholme, Philip
    Date
    2018
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Tapadia, M. and Carlessi, R. and Johnson, S. and Utikar, R. and Newsholme, P. 2018. Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells. Molecular and Cellular Endocrinology. 480: pp. 83-96.
    Source Title
    Molecular and Cellular Endocrinology
    DOI
    10.1016/j.mce.2018.10.015
    ISSN
    0303-7207
    School
    School of Pharmacy and Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/71848
    Collection
    • Curtin Research Publications
    Abstract

    Lupin seed proteins have been reported to exhibit hypoglycaemic effects in animals and humans following oral administration, however little is known about its mechanism of action. This study investigated the signalling pathway(s) responsible for the insulinotropic effect of the hydrolysate obtained from lupin (Lupinus angustifolius L.) seed extracts utilizing BRIN-BD11 ß-cells. The extract was treated with digestive enzymes to give a hydrolysate rich in biomolecules =7 kDa. Cells exhibited hydrolysate induced dose-dependent stimulation of insulin secretion and enhanced intracellular Ca2+ and glucose metabolism. The stimulatory effect of the hydrolysate was potentiated by depolarizing concentrations of KCl and was blocked by inhibitors of the ATP sensitive K+ channel, Gaq protein, phospholipase C (PLC) and protein kinase C (PKC). These findings reveal a novel mechanism for lupin hydrolysate stimulated insulin secretion via Gaq mediated signal transduction (Gaq/PLC/PKC) in the ß-cells. Thus, lupin hydrolysates may have potential for nutraceutical treatment in type 2 diabetes.

    Related items

    Showing items related by title, author, creator and subject.

    • Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds
      Tapadia, Mrunmai; Johnson, Stuart ; Utikar, Ranjeet ; Newsholme, Philip ; Carlessi, Rodrigo (2021)
      The glucose modulating properties of lupin have been attributed to its seed protein γ-conglutin. Here we explored the antidiabetic potential of γ-conglutin purified from lupin seeds in-vitro. To mimic the effects of an ...
    • Anti-diabetic action of Lupinus angustifolius seed proteins and peptides
      Tapadia, Mrunmai (2019)
      Lupin seed proteins have been reported to exhibit hypoglycaemic action in animals and humans. This research focuses on exploring in-vitro anti-diabetic action of proteins extracted from Lupinus angustifolius seed. A novel ...
    • Casein Hydrolysate with Glycemic Control Properties: Evidence from Cells, Animal Models, and Humans
      Drummond, E.; Flynn, S.; Whelan, H.; Nongonierma, A.; Holton, T.; Robinson, A.; Egan, T.; Cagney, G.; Shields, D.; Gibney, E.; Newsholme, Philip; Gaudel, C.; Jacquier, J.; Noronha, N.; Fitzgerald, R.; Brennan, L. (2018)
      © 2018 American Chemical Society. Evidence exists to support the role of dairy derived proteins whey and casein in glycemic management. The objective of the present study was to use a cell screening method to identify a ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.