Depth-dependent refractive index of normal and early degenerated articular cartilage
MetadataShow full item record
This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.
The depth distributions of refractive index (RI) in normal and early degenerated articular cartilage (AC) were measured and correlated to the pathological statuses. Utilizing a confocal microscope, the depth distribution of RI was determined with an interval of 50µm from the articular surface and approximated as a smooth curve. The fluctuation of RI was quantified with a specially defined coefficient and the proteoglycan (PG) loss was quantified with histological images. The overall RI of the AC collected from the femoral condyles of mature sheep was 1.4444. Significant differences existed in the refractive indices of different cartilage samples or even in the different depths of the same sample. The RI of normal cartilage distributed as a regular ramp or arch curve with depth and peaked in the middle-deep zone. The cartilage samples detected with high PG losses were coupled with severe fluctuations of RI. The fluctuating level was increased with pathological progress. The coefficient of RI fluctuation can distinguish the normal cartilage from the initially degenerated one, which potentially provides an indicator to detect the pathological development of AC.
Showing items related by title, author, creator and subject.
Characterizing depth-dependent refractive index of articular cartilage subjected to mechanical wear or enzymic degenerationWang, K.; Wu, Jian-Ping; Day, R.; Kirk, Brett; Hu, X. (2016)Utilizing a laser scanning confocal microscope system, the refractive indices of articular cartilage (AC) with mechanical or biochemical degenerations were characterized to investigate whether potential correlations exist ...
Wang, K.; Wu, Ping; Day, R.; Kirk, Thomas Brett (2012)This study proposes a method for measuring the refractive index of articular cartilage within a thin and small specimen slice. The cartilage specimen, with a thickness of about 50 μm, was put next to a thin film of immersion ...
Wu, Ping; Kirk, Brett; Milne, N.; Delaney, P. (2008)Osteoarthritis is synonymous to the functional disorder of articular cartilage. Mechanical tests of articular cartilage offer a way to understand the tissue’s functionality and osteoarthritis. During normal activities, ...