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    Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections

    Access Status
    Fulltext not available
    Authors
    Alfaro-Núñez, A.
    Gilbert, Thomas
    Date
    2014
    Type
    Journal Article
    
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    Citation
    Alfaro-Núñez, A. and Gilbert, T. 2014. Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. Journal of Virological Methods. 206: pp. 38-41.
    Source Title
    Journal of Virological Methods
    DOI
    10.1016/j.jviromet.2014.05.019
    ISSN
    0166-0934
    URI
    http://hdl.handle.net/20.500.11937/12708
    Collection
    • Curtin Research Publications
    Abstract

    The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.

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