Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections
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The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.
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Global distribution of Chelonid fibropapilloma-associated herpesvirus among clinically healthy sea turtlesAlfaro-Nunez, A.; Bertelsen, M.; Bojesen, A.; Rasmussen, I.; Zepeda-Mendoza, L.; Olsen, M.; Gilbert, Thomas (2014)Background: Fibropapillomatosis (FP) is a neoplastic disease characterized by cutaneous tumours that has been documented to infect all sea turtle species. Chelonid fibropapilloma-associated herpesvirus (CFPHV) is believed ...
Further evidence of chelonid herpesvirus 5 (ChHV5) latency: High levels of ChHV5 DNA detected in clinically healthy marine turtlesAlfaro-Nunez, A.; Bojesen, A.; Bertelsen, M.; Wales, N.; Balazs, G.; Gilbert, Thomas (2016)The Chelonid herpesvirus 5 (ChHV5) has been consistently associated with fibropapillomatosis (FP), a transmissible neoplastic disease of marine turtles. Whether ChHV5 plays a causal role remains debated, partly because ...
Gagnon, Marthe Monique; Rawson, Christopher (2010)• Necropsies were collected from a green turtle (Chelonia mydas) collected from Ashmore Reef in November 2009.• A total of eleven samples (4 muscle tissue samples, 2 gut content samples and 5 swab samples from external ...