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dc.contributor.authorAlfaro-Núñez, A.
dc.contributor.authorGilbert, Thomas
dc.date.accessioned2017-01-30T11:32:19Z
dc.date.available2017-01-30T11:32:19Z
dc.date.created2014-06-29T20:00:19Z
dc.date.issued2014
dc.identifier.citationAlfaro-Núñez, A. and Gilbert, T. 2014. Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. Journal of Virological Methods. 206: pp. 38-41.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/12708
dc.identifier.doi10.1016/j.jviromet.2014.05.019
dc.description.abstract

The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.

dc.publisherElsevier BV
dc.subjectLatency
dc.subjectChelonid fibropapilloma-associated herpesvirus
dc.subjectSensitivity
dc.subjectSea turtles
dc.subjectFibropapillomatosis
dc.subjectPCR
dc.titleValidation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections
dc.typeJournal Article
dcterms.source.volume206
dcterms.source.startPage38
dcterms.source.endPage41
dcterms.source.issn0166-0934
dcterms.source.titleJournal of Virological Methods
curtin.department
curtin.accessStatusFulltext not available


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