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dc.contributor.authorPodolska, Anna
dc.contributor.authorTham, S.
dc.contributor.authorHart, Robert
dc.contributor.authorSeeber, R.
dc.contributor.authorKocan, M.
dc.contributor.authorKocan, M.
dc.contributor.authorMishra, U.
dc.contributor.authorPfleger, K.
dc.contributor.authorParish, G.
dc.contributor.authorNener, B.
dc.date.accessioned2017-01-30T11:34:06Z
dc.date.available2017-01-30T11:34:06Z
dc.date.created2014-04-06T20:00:38Z
dc.date.issued2012
dc.identifier.citationPodolska, Anna and Tham, Stephanie and Hart, Robert D. and Seeber, Ruth M. and Kocan, Martin and Kocan, Martina and Mishra, Umesh K. and Pfleger, Kevin D.G. and Parish, Gia and Nener, Brett D. 2012. Biocompatibility of semiconducting AlGaN/GaN material with living cells. Sensors and Actuators B: Chemical. 169 (5): pp. 401-406.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/12988
dc.identifier.doi10.1016/j.snb.2012.04.015
dc.description.abstract

With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications.

dc.publisherElsevier SA
dc.subjectBiocompatibility
dc.subjectCell/semiconductor interface
dc.subjectAlGaN/GaN
dc.subjectCell-based biosensor
dc.subjectHEK293
dc.titleBiocompatibility of semiconducting AlGaN/GaN material with living cells
dc.typeJournal Article
dcterms.source.volume169
dcterms.source.startPage401
dcterms.source.endPage406
dcterms.source.issn09254005
dcterms.source.titleSensors and Actuators B
curtin.department
curtin.accessStatusFulltext not available


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