Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
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A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transformation vector was constructed which contains this marker and confers HmB resistance to Aspergillus species.With both A. niger and A. nidulans, transformation frequencies of 5–20 transformants per μg vector DNA were obtained. Cotransformation with other vectors was shown to be very efficient in both species, when selection for HmB resistance was applied.
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Tunlid, A.; Ahman, J.; Oliver, Richard (1999)The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. ...
Oliver, Richard; Roberts, I.; Harling, R.; Kenyon, L.; Punt, P.; Dingemanse, M.; Van Den Hondel, C. (1987)A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase ...
Farman, M.; Oliver, Richard (1988)Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an ...