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    Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli

    Access Status
    Fulltext not available
    Authors
    Punt, P.
    Oliver, Richard
    Dingemanse, M.
    Pouwels, P.
    Van Den Hondel, C.
    Date
    1987
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    PUNT PJ, OLIVER RP, DINGEMANSE MA, POUWELS PH and VAN DEN HONDEL CAMJJ (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56 117-124
    DOI
    10.1016/0378-1119(87)90164-8
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/14903
    Collection
    • Curtin Research Publications
    Abstract

    A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transformation vector was constructed which contains this marker and confers HmB resistance to Aspergillus species.With both A. niger and A. nidulans, transformation frequencies of 5–20 transformants per μg vector DNA were obtained. Cotransformation with other vectors was shown to be very efficient in both species, when selection for HmB resistance was applied.

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