Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
| dc.contributor.author | Punt, P. | |
| dc.contributor.author | Oliver, Richard | |
| dc.contributor.author | Dingemanse, M. | |
| dc.contributor.author | Pouwels, P. | |
| dc.contributor.author | Van Den Hondel, C. | |
| dc.date.accessioned | 2017-01-30T11:46:39Z | |
| dc.date.available | 2017-01-30T11:46:39Z | |
| dc.date.created | 2010-11-12T04:02:39Z | |
| dc.date.issued | 1987 | |
| dc.identifier.citation | PUNT PJ, OLIVER RP, DINGEMANSE MA, POUWELS PH and VAN DEN HONDEL CAMJJ (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56 117-124 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/14903 | |
| dc.identifier.doi | 10.1016/0378-1119(87)90164-8 | |
| dc.description.abstract |
A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transformation vector was constructed which contains this marker and confers HmB resistance to Aspergillus species.With both A. niger and A. nidulans, transformation frequencies of 5–20 transformants per μg vector DNA were obtained. Cotransformation with other vectors was shown to be very efficient in both species, when selection for HmB resistance was applied. | |
| dc.title | Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli | |
| dc.type | Journal Article | |
| curtin.note |
A copy of this item may be available from Professor Richard Oliver | |
| curtin.note |
Email: Richard.oliver@curtin.edu.au | |
| curtin.accessStatus | Fulltext not available | |
| curtin.faculty | Department of Environmental & Agriculture | |
| curtin.faculty | School of Agriculture and Environment | |
| curtin.faculty | Faculty of Science and Engineering |