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    Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes

    Access Status
    Fulltext not available
    Authors
    Mooranian, Armin
    Negrulj, Rebecca
    Jamieson, E.
    Morahan, G.
    Al-Salami, Hani
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Mooranian, A. and Negrulj, R. and Jamieson, E. and Morahan, G. and Al-Salami, H. 2016. Biological assessments of encapsulated pancreatic ß-Cells: Their potential transplantation in diabetes. Cellular and Molecular Bioengineering. [In Press].
    Source Title
    Cellular and Molecular Bioengineering
    DOI
    10.1007/s12195-016-0441-z
    ISSN
    1865-5025
    School
    School of Pharmacy
    URI
    http://hdl.handle.net/20.500.11937/15066
    Collection
    • Curtin Research Publications
    Abstract

    © 2016. Biomedical Engineering Society. Microencapsulation of pancreatic islets has been considered as a promising method for cell transplantation and diabetes treatment. However, in vivo trials to date have been hampered by fibrotic overgrowth and very limited to no success, long-term. Future success requires suitable microencapsulating method and possibly a simplified and suitable formulation which will produce a microcapsule that provides an immunobarrier, maintain full ß-cell functionality whilst also reducing the inflammatory processes that induce fibrosis. In multiple studies, we screened various formulations and microencapsulating methods, and obtained promising results using bile acid-based microcapsules containing ß-cells, in terms of cell functions and insulin release. Thus, this study aimed to refine further the microencapsulating method using a simple alginate-poly-l-ornithine formulation and test the effect of adding a promising bile acid, ursodeoxycholic acid (UDCA), on cell functions. Using Büchi concentric nozzle, viable NIT-1 cells were microencapsulated using alginate-poly-l-ornithine, with or without UDCA at a ratio of 1:1.2 or 1:1.2:4. Screening for nozzle temperature and nozzle-gelation bath distance was carried out to form best microcapsules. Microcapsules were cultured for 48 h and examined for size and surface morphology, chemical profiling and ß-cell viability. Culture supernatants were examined for insulin and inflammatory cytokines. When using 30 °C nozzle-temperature and 5 cm nozzle-gelation bath distance, in the presence of the bile acid, cell mitochondrial activities and insulin production were optimised. Under deployed microencapsulating method with nozzle-temperature of 30 °C and nozzle-gelation bath distance of 5 cm, the incorporation of the bile acid into the microcapsules resulted in enhanced ß-cell survival, function and improved overall biocompatibility supporting potential applications in transplantation.

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    • Alginate-deoxycholic Acid Interaction and Its Impact on Pancreatic ?-Cells and Insulin Secretion and Potential Treatment of Type 1 Diabetes
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      © 2016. Springer Science+Business Media New York.Introduction: The secondary bile acid, deoxycholic acid (DCA), has been shown to exert membrane stabilising effects on a pH sensitive delivery system for the oral delivery ...
    • Primary Bile Acid Chenodeoxycholic Acid-Based Microcapsules to Examine ß-cell Survival and the Inflammatory Response
      Mooranian, A.; Negrulj, R.; Al-Salami, Hani (2016)
      In past studies using hydrogel-polyelectrolyte matrix and different bile acid excipients, we microencapsulated pancreatic ß-cells using various methods, and the microcapsules were mechanically stable, displayed good ...
    • Influence of Biotechnological Processes, Speed of Formulation Flow and Cellular Concurrent Stream-Integration on Insulin Production from ß-cells as a Result of Co-Encapsulation with a Highly Lipophilic Bile Acid
      Mooranian, A.; Negrulj, R.; Takechi, Ryu; Jamieson, E.; Morahan, G.; Al-Salami, Hani (2017)
      Introduction: We have shown that incorporation of the hydrophilic bile acid, ursodeoxycholic acid, into ß-cell microcapsules exerted positive effects on microcapsules’ morphology and size, but these effects were excipient ...
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