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    A comparative analysis of the heterotrimeric G-protein G[alpha], G[beta] and G[gamma] subunits in the wheat pathogen Stagonospora nodorum

    218336_67484_1471-2180-12-131.pdf (2.285Mb)
    Access Status
    Open access
    Authors
    Gummer, J.
    Trengove, R.
    Oliver, Richard
    Solomon, P.
    Date
    2012
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Gummer, J. and Trengove, R. and Oliver, R. and Solomon, P. 2012. A comparative analysis of the heterotrimeric G-protein G[alpha], G[beta] and G[gamma] subunits in the wheat pathogen Stagonospora nodorum. BMC Microbiology. 12: Article ID 131.
    Source Title
    BMC Microbiology
    DOI
    10.1186/1471-2180-12-131
    ISSN
    1471-2180
    School
    Department of Environment and Agriculture
    Remarks

    This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons License http://creativecommons.org/licenses/by/2.0/. Please refer to the licence to obtain terms for any further reuse or distribution of this work.

    URI
    http://hdl.handle.net/20.500.11937/27942
    Collection
    • Curtin Research Publications
    Abstract

    Background: It has been well established that the Gα subunit of the heterotrimeric G-protein in the wheat pathogen Stagonospora nodorum is required for a variety of phenotypes including pathogenicity, melanisation and asexual differentiation. The roles though of the Gγ and Gβ subunits though were unclear. The objective of this study was to identify and understand the role of these subunits and assess their requirement for pathogenicity and development. Results: G-protein Gγ and Gβ subunits, named Gga1 and Gba1 respectively, were identified in the Stagonospora nodorum genome by comparative analysis with known fungal orthologues. A reverse genetics technique was used to study the role of these and revealed that the mutant strains displayed altered in vitro growth including a differential response to a variety of exogenous carbon sources. Pathogenicity assays showed that Stagonospora nodorum strains lacking Gba1 were essentially non-pathogenic whilst Gga1-impaired strains displayed significantly slower growth in planta. Subsequent sporulation assays showed that like the previously described Gα subunit mutants, both Gba1 and Gga1 were required for asexual sporulation with neither mutant strain being able to differentiate either pycnidia nor pycnidiospores under normal growth conditions. Continued incubation at 4°C was found to complement the mutation in each of the G-protein subunits with nearly wild-type levels of pycnidia recovered. Conclusion: This study provides further evidence on the significance of cAMP-dependent signal transduction for many aspects of fungal development and pathogenicity. The observation that cold temperatures can complement the G-protein sporulation defect now provides an ideal tool by which asexual differentiation can now be dissected.

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    • Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum
      Gummer, J.; Trengove, R.; Oliver, Richard; Solomon, P. (2013)
      Mutants of the wheat pathogenic fungus Stagonospora nodorum lacking G-protein subunits display a variety of phenotypes including melanization defects, primary metabolic changes and a decreased ability to sporulate. To ...
    • Quantitative proteomic analysis of G-protein signalling in Stagonospora nodorum using isobaric tags for relative and absolute quantification
      Casey, T.; Solomon, P.; Bringans, S.; Tan, Kar-Chun; Oliver, Richard; Lipscombe, R. (2010)
      The G protein α-subunit (Gna1) in the wheat pathogen Stagonospora nodorum has previously been shown to be a critical controlling element in disease ontogeny. In this study, iTRAQ and 2-D LC MALDI-MS/MS have been used to ...
    • Quantitative proteomic analysis of G-protein signallingin Stagonospora nodorum using isobaric tags forrelative and absolute quantification
      Casey, T.; Solomon, P.; Bringans, S.; Tan, Kar-Chun; Oliver, Richard; Lipscombe, R. (2010)
      The G protein a-subunit (Gna1) in the wheat pathogen Stagonospora nodorum has previouslybeen shown to be a critical controlling element in disease ontogeny. In this study, iTRAQ and2-D LC MALDI-MS/MS have been used to ...
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