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    An integrated microspectrometer for localised multiplexing measurements

    233213_233213.pdf (4.737Mb)
    Access Status
    Open access
    Authors
    Hu, Z.
    Glidle, A.
    Ironside, Charlie
    Cooper, J.
    Yin, H.
    Date
    2015
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Hu, Z. and Glidle, A. and Ironside, C. and Cooper, J. and Yin, H. 2015. An integrated microspectrometer for localised multiplexing measurements. Lab on a Chip: Miniaturisation for Chemistry and Biology. 15 (1): pp. 283-289.
    Source Title
    Lab on a Chip - Miniaturisation for Chemistry and Biology
    DOI
    10.1039/c4lc00952e
    ISSN
    1473-0197
    School
    Department of Physics and Astronomy
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/3.0/

    URI
    http://hdl.handle.net/20.500.11937/30681
    Collection
    • Curtin Research Publications
    Abstract

    We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location. This journal is

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