Giardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections
MetadataShow full item record
In view of the interest in genotype-specific pathogenesis in Giardia duodenalis, the aim of the present study was to examine the effects of infection with different, or mixed, G. duodenalis assemblages on the integrity of human intestinal epithelia. To that end, human epithelial cells (HCT-8) were cultured and exposed to different G. duodenalis assemblages (A-B-E) or a combination of these assemblages. Epithelial disruption and apoptosis were evaluated by fluorescent microscopy and apoptotic oligonucleosome quantification. The results indicate that infection with trophozoites disrupts epithelialtight junctions and induces varying degrees of enterocyte apoptosis depending on the infecting assemblage. All disruptions were caspase-3 dependent, and were more pronounced when caused by a non-host specific assemblage. Furthermore, infections by isolates in combination with isolates from another assemblage enhanced the epithelial disruption and apoptosis. Further studies in vitro and in vivo are required to confirm the mechanisms of enhanced pathogenicity of mixed, and/or non-host specific, G. duodenalis infections. Findings in the present study point to the potential pathogenicimportance of intra-species polyparasitism in giardiasis.
Showing items related by title, author, creator and subject.
Kicic, Anthony; Stevens, P.; Sutanto, E.; Kicic-Starcevich, E.; Ling, K.; Looi, K.; Martinovich, K.; Garratt, L.; Iosifidis, T.; Shaw, N.; Buckley, A.; Rigby, P.; Lannigan, F.; Knight, D.; Stick, S. (2016)© 2016 John Wiley & Sons Ltd Background: The airway epithelium forms an effective immune and physical barrier that is essential for protecting the lung from potentially harmful inhaled stimuli including viruses. Human ...
Effect of human rhinovirus infection on airway epithelium tight junction protein disassembly and transepithelial permeabilityLooi, K.; Troy, N.; Garratt, L.; Iosifidis, T.; Bosco, A.; Buckley, A.; Ling, K.; Martinovich, K.; Kicic-Starcevich, E.; Shaw, N.; Sutanto, E.; Zosky, G.; Rigby, P.; Larcombe, Alexander; Knight, D.; Kicic, Anthony; Stick, S. (2016)© 2016 Taylor & Francis. Rationale: No studies have assessed the effects of human rhinovirus (HRV) infection on epithelial tight junctions (TJs) and resultant barrier function. Aim of the Study: To correlate viral ...
Trend, S.; Chang, B.J.; O'Dea, M.; Stick, S.M.; Kicic, Anthony (2018)© 2007 - 2018 Frontiers Media S.A. All Rights Reserved. Antimicrobial-resistant microbes are an increasing threat to human health. In cystic fibrosis (CF), airway infections with Pseudomonas aeruginosa remain a key driver ...