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dc.contributor.authorKoh, W.
dc.contributor.authorGeurden, T.
dc.contributor.authorPaget, T.
dc.contributor.authorO'Handley, R.
dc.contributor.authorSteuart, Robert
dc.contributor.authorThompson, R.
dc.contributor.authorBuret, A.
dc.date.accessioned2017-01-30T13:26:05Z
dc.date.available2017-01-30T13:26:05Z
dc.date.created2013-03-12T20:00:32Z
dc.date.issued2012
dc.identifier.citationKoh, Wan Hon and Geurden, Thomas and Paget, Tim and O'Handley, Ryan and Steuart, Robert F. and Thompson, R.C. Andrew and Buret, Andre G. 2013. Giardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections. Journal of Parasitology 99 (2) : pp. 353-358.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/31545
dc.identifier.doi10.1645/GE-3021.1
dc.description.abstract

In view of the interest in genotype-specific pathogenesis in Giardia duodenalis, the aim of the present study was to examine the effects of infection with different, or mixed, G. duodenalis assemblages on the integrity of human intestinal epithelia. To that end, human epithelial cells (HCT-8) were cultured and exposed to different G. duodenalis assemblages (A-B-E) or a combination of these assemblages. Epithelial disruption and apoptosis were evaluated by fluorescent microscopy and apoptotic oligonucleosome quantification. The results indicate that infection with trophozoites disrupts epithelialtight junctions and induces varying degrees of enterocyte apoptosis depending on the infecting assemblage. All disruptions were caspase-3 dependent, and were more pronounced when caused by a non-host specific assemblage. Furthermore, infections by isolates in combination with isolates from another assemblage enhanced the epithelial disruption and apoptosis. Further studies in vitro and in vivo are required to confirm the mechanisms of enhanced pathogenicity of mixed, and/or non-host specific, G. duodenalis infections. Findings in the present study point to the potential pathogenicimportance of intra-species polyparasitism in giardiasis.

dc.publisherAmerican Society of Parasitologists
dc.relation.urihttp://www.journalofparasitology.org/
dc.titleGiardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections
dc.typeJournal Article
dcterms.source.volumel
dcterms.source.startPagel
dcterms.source.endPage14
dcterms.source.issn0022-3395
dcterms.source.titleJournal of Parasitology
curtin.department
curtin.accessStatusFulltext not available


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