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    Quantitative Real-Time PCR in aDNA Research

    Access Status
    Fulltext not available
    Authors
    Bunce, Michael
    Oskam, C.
    Allentoft, M.
    Date
    2012
    Type
    Book Chapter
    
    Metadata
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    Citation
    Bunce, M. and Oskam, C. and Allentoft, M. 2012. Quantitative Real-Time PCR in aDNA Research, in Shapiro, Beth; Hofreiter, Michael (ed), Ancient DNA: Methods and Protocols, pp. 121-132. New York: Humana Press.
    Source Title
    Ancient DNA: Methods and Protocols
    DOI
    10.1007/978-1-61779-516-9_16
    ISBN
    978-1-61779-515-2
    URI
    http://hdl.handle.net/20.500.11937/3212
    Collection
    • Curtin Research Publications
    Abstract

    Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing effi cient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates.

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