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dc.contributor.authorBunce, Michael
dc.contributor.authorOskam, C.
dc.contributor.authorAllentoft, M.
dc.contributor.editorShapiro, Beth; Hofreiter, Michael
dc.date.accessioned2017-01-30T10:29:26Z
dc.date.available2017-01-30T10:29:26Z
dc.date.created2014-09-02T20:01:16Z
dc.date.issued2012
dc.identifier.citationBunce, M. and Oskam, C. and Allentoft, M. 2012. Quantitative Real-Time PCR in aDNA Research, in Shapiro, Beth; Hofreiter, Michael (ed), Ancient DNA: Methods and Protocols, pp. 121-132. New York: Humana Press.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/3212
dc.identifier.doi10.1007/978-1-61779-516-9_16
dc.description.abstract

Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing effi cient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates.

dc.publisherHumana Press
dc.subjectqPCR standard
dc.subjectLibrary quantitation
dc.subjectPCR inhibition
dc.subjectAncient DNA
dc.subjectDNA extraction optimization
dc.subjectqPCR
dc.subjectSYBR Green
dc.titleQuantitative Real-Time PCR in aDNA Research
dc.typeBook Chapter
dcterms.source.volume840
dcterms.source.startPage121
dcterms.source.endPage132
dcterms.source.titleAncient DNA: Methods and Protocols
dcterms.source.isbn978-1-61779-515-2
dcterms.source.placeUnited States
dcterms.source.chapter24
curtin.accessStatusFulltext not available


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