Quantitative Real-Time PCR in aDNA Research
| dc.contributor.author | Bunce, Michael | |
| dc.contributor.author | Oskam, C. | |
| dc.contributor.author | Allentoft, M. | |
| dc.contributor.editor | Shapiro | |
| dc.contributor.editor | Beth | |
| dc.contributor.editor | Hofreiter | |
| dc.contributor.editor | Michael | |
| dc.date.accessioned | 2017-01-30T10:29:26Z | |
| dc.date.available | 2017-01-30T10:29:26Z | |
| dc.date.created | 2014-09-02T20:01:16Z | |
| dc.date.issued | 2012 | |
| dc.identifier.citation | Bunce, M. and Oskam, C. and Allentoft, M. 2012. Quantitative Real-Time PCR in aDNA Research, in Shapiro, Beth; Hofreiter, Michael (ed), Ancient DNA: Methods and Protocols, pp. 121-132. New York: Humana Press. | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/3212 | |
| dc.identifier.doi | 10.1007/978-1-61779-516-9_16 | |
| dc.description.abstract |
Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing effi cient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates. | |
| dc.publisher | Humana Press | |
| dc.subject | qPCR standard | |
| dc.subject | Library quantitation | |
| dc.subject | PCR inhibition | |
| dc.subject | Ancient DNA | |
| dc.subject | DNA extraction optimization | |
| dc.subject | qPCR | |
| dc.subject | SYBR Green | |
| dc.title | Quantitative Real-Time PCR in aDNA Research | |
| dc.type | Book Chapter | |
| dcterms.source.volume | 840 | |
| dcterms.source.startPage | 121 | |
| dcterms.source.endPage | 132 | |
| dcterms.source.title | Ancient DNA: Methods and Protocols | |
| dcterms.source.isbn | 978-1-61779-515-2 | |
| dcterms.source.place | United States | |
| dcterms.source.chapter | 24 | |
| curtin.accessStatus | Fulltext not available |