Modulation of the organelle specificity in Re(i) tetrazolato complexes leads to labeling of lipid droplets
|dc.identifier.citation||Bader, Christie A. and Brooks, Robert D. and Ng, Yeap S. and Sorvina, Alexandra and Werrett, Melissa V. and Wright, Phillip J. and Anwer, Ayad G. and Brooks, Douglas A. and Stagni, Stefano and Muzzioli, Sara and Silberstein, Morry and Skelton, Brian W. and Goldys, Ewa M. and Plush, Sally E. and Shandala, Tetyana and Massi, Massimiliano. 2014. Modulation of the organelle specificity in Re(I) tetrazolato complexes leads to labeling of lipid droplets. RSC Advances. 4 (31): pp. 16345-16351.|
The biological behaviour in terms of cellular incubation and organelle specificity for two complexes of the type fac-[Re(CO)3(phen)L], where phen is 1,10-phenanthroline and L is either 3-pyridyltetrazolate or4-cyanophenyltetrazolate, are herein investigated. The emission signal detected from the live insect Drosophila and human cell lines, generated by exploiting two-photon excitation at 830 nm to reduce cellular damage and autofluorescence, suggests photophysical properties that are analogous to those measured from dilute solutions, meaning that the complexes remain intact within the cellular environment. Moreover, the rhenium complex linked to 4-cyanophenyltetrazolateshows high specificity for the lipid droplets, whereas the complex bound to 3-pyridyltetrazolate tends to localise within the lysosomes. This differential localisation implies that in thesecomplexes, organelle specificity can be achieved and manipulated by simple functional group transformations thus avoiding more complex bioconjugation strategies. More importantly, these results highlight the first example of phosphorescent labeling of the lipid droplets, whose important cellular functions have been recently highlighted along with the fact that their role in the metabolism of healthy and diseased cells has not been fully elucidated.
|dc.publisher||Royal Society of Chemistry|
|dc.title||Modulation of the organelle specificity in Re(i) tetrazolato complexes leads to labeling of lipid droplets|