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    Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

    Access Status
    Fulltext not available
    Authors
    Chumbimuni-Torres, K.
    Wu, J.
    Clawson, C.
    Galik, M.
    Walter, A.
    Flechsig, G.
    Bakker, Eric
    Zhang, L.
    Wang, J.
    Date
    2010
    Type
    Journal Article
    
    Metadata
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    Citation
    Chumbimuni-Torres, Karin Y. and Wu, Jie and Clawson, Corbin and Galik, Michal and Walter, Anne and Flechsig, Gerd-Uwe and Bakker, Eric and Zhang, Liangfang and Wang, Joseph. 2010. Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes. Analyst. 135 (7): pp. 1618-1623.
    Source Title
    Analyst
    DOI
    10.1039/c0an00198h
    ISSN
    0003-2654
    School
    Nanochemistry Research Institute (Research Institute)
    URI
    http://hdl.handle.net/20.500.11937/37024
    Collection
    • Curtin Research Publications
    Abstract

    Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of ‘green’ calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays.

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