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dc.contributor.authorChumbimuni-Torres, K.
dc.contributor.authorWu, J.
dc.contributor.authorClawson, C.
dc.contributor.authorGalik, M.
dc.contributor.authorWalter, A.
dc.contributor.authorFlechsig, G.
dc.contributor.authorBakker, Eric
dc.contributor.authorZhang, L.
dc.contributor.authorWang, J.
dc.date.accessioned2017-01-30T13:59:03Z
dc.date.available2017-01-30T13:59:03Z
dc.date.created2011-06-21T20:01:34Z
dc.date.issued2010
dc.identifier.citationChumbimuni-Torres, Karin Y. and Wu, Jie and Clawson, Corbin and Galik, Michal and Walter, Anne and Flechsig, Gerd-Uwe and Bakker, Eric and Zhang, Liangfang and Wang, Joseph. 2010. Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes. Analyst. 135 (7): pp. 1618-1623.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/37024
dc.identifier.doi10.1039/c0an00198h
dc.description.abstract

Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of ‘green’ calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays.

dc.publisherRoyal Society of Chemistry
dc.titleAmplified potentiometric transduction of DNA hybridization using ion-loaded liposomes
dc.typeJournal Article
dcterms.source.volume135
dcterms.source.number7
dcterms.source.startPage1618
dcterms.source.endPage1623
dcterms.source.issn0003-2654
dcterms.source.titleAnalyst
curtin.departmentNanochemistry Research Institute (Research Institute)
curtin.accessStatusFulltext not available


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