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dc.contributor.authorTan, K.
dc.contributor.authorHeazlewood, J.
dc.contributor.authorMillar, A.
dc.contributor.authorThomson, G.
dc.contributor.authorOliver, Richard
dc.contributor.authorSolomon, P.
dc.date.accessioned2017-01-30T14:34:23Z
dc.date.available2017-01-30T14:34:23Z
dc.date.created2012-01-18T07:57:16Z
dc.date.issued2008
dc.identifier.citationTan, Kar-Chun and Heazlewood, Joshua L. and Millar, A. Harvey, and Thomson, Gordon and Oliver, Richard P. and Solomon, Peter S. 2008. A Signaling-Regulated, Short-Chain Dehydrogenase of Stagonospora nodorum Regulates Asexual Development. Eukaryotic Cell. 7 (11): pp. 1916-1929.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/39493
dc.identifier.doi10.1128/EC.00237-08
dc.description.abstract

The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development defects and reduced pathogenicity [P. S. Solomon et al., Mol. Plant-Microbe Interact. 17: 456-466, 2004]. In this study, we sought to identify targets of the signaling pathway that may have contributed to phenotypic defects of the signaling mutants. A comparative analysis of Stagonospora nodorum wild-type and G alpha-defective mutant (gna1) intracellular proteomes was performed via two-dimensional polyacrylamide gel electrophoresis. Several proteins showed significantly altered abundances when comparing the two strains. One such protein, the short-chain dehydrogenase Sch1, was 18-fold less abundant in the gna1 strain, implying that it is positively regulated by G alpha signaling. Gene expression and transcriptional enhanced green fluorescent protein fusion analyses of Sch1 indicates strong expression during asexual development. Mutant strains of Stagonospora nodorum lacking Sch1 demonstrated poor growth on minimal media and exhibited a significant reduction in asexual sporulation on all growth media examined. Detailed histological experiments on sch1 pycnidia revealed that the gene is required for the differentiation of the subparietal layers of asexual pycnidia resulting in a significant reduction in both pycnidiospore size and numbers.

dc.publisherAmerican Society for Microbiology
dc.subjectElectron Microscopy
dc.subjectAppressorium Formation
dc.subjectMycosphaerella-Graminicola
dc.subjectIn-Vitro
dc.subjectFungus Magnaporthe-Grisea
dc.subjectBotrytis-Cinerea
dc.subjectFusarium-Graminearum
dc.subjectUstilago-Maydis
dc.subjectProteomic Analysis
dc.subjectProtein Alpha-Subunit
dc.titleA Signaling-Regulated, Short-Chain Dehydrogenase of Stagonospora nodorum Regulates Asexual Development
dc.typeJournal Article
dcterms.source.volume7
dcterms.source.number11
dcterms.source.startPage1916
dcterms.source.endPage1929
dcterms.source.issn1535-9778
dcterms.source.titleEukaryotic Cell
curtin.departmentDepartment of Environment and Agriculture
curtin.accessStatusOpen access


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