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    Optimisation of batch culture conditions for cell-envelope-associated proteinase production from lactobacillus delbrueckii subsp. lactis ATCC® 7830™

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    Authors
    Agyei, D.
    Potumarthi, R.
    Danquah, Michael
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    Agyei, D. and Potumarthi, R. and Danquah, M. 2012. Optimisation of batch culture conditions for cell-envelope-associated proteinase production from lactobacillus delbrueckii subsp. lactis ATCC® 7830™. Applied Biochemistry and Biotechnology. 168 (5): pp. 1035-1050.
    Source Title
    Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
    DOI
    10.1007/s12010-012-9839-9
    ISSN
    0273-2289
    School
    Curtin Sarawak
    URI
    http://hdl.handle.net/20.500.11937/41638
    Collection
    • Curtin Research Publications
    Abstract

    Using a combination of conventional sequential techniques, the batch growth conditions for the production of cell-envelope-associated proteinases have for the first time been studied and optimised in Lactobacillus delbrueckii subsp. lactis 313 (ATCC 7830; LDL 313). Concentrations of inoculum (0.1 < X < 10 % vol/vol), agitation speed (0 < S < 200 rpm), varying incubation temperature (30 < T < 50 °C), starting pH (4.5 < pH < 7) and carbon/nitrogen ratio of production medium (0.2 < r < 5) had an individual effect on proteinase yield (p < 0.01). Optimal conditions for proteinase production included an initial pH of 6.0, 45 °C incubation temperature, 2 % (v/v) inoculum size of OD560 = 1, 150 rpm agitation speed, and growth medium carbon/nitrogen ratio of 1.0. Maximum proteinase activity obtained for whole cells was 0.99 U/ml after 8 h of incubation. The variables studied are very relevant due to their significance in improving the productivity of proteinase synthesis from LDL 313, under process and, likely, economic optimum conditions.

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      This chapter deals with the downstream processes of lactobacilli proteinases recovery, isolation and purification strategies. One of the most pressing bioprocess challenges in the area of production of pharmaceutical/biological ...
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