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dc.contributor.authorAgyei, D.
dc.contributor.authorPotumarthi, R.
dc.contributor.authorDanquah, Michael
dc.date.accessioned2017-01-30T14:53:50Z
dc.date.available2017-01-30T14:53:50Z
dc.date.created2016-06-05T19:31:10Z
dc.date.issued2012
dc.identifier.citationAgyei, D. and Potumarthi, R. and Danquah, M. 2012. Optimisation of batch culture conditions for cell-envelope-associated proteinase production from lactobacillus delbrueckii subsp. lactis ATCC® 7830™. Applied Biochemistry and Biotechnology. 168 (5): pp. 1035-1050.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/41638
dc.identifier.doi10.1007/s12010-012-9839-9
dc.description.abstract

Using a combination of conventional sequential techniques, the batch growth conditions for the production of cell-envelope-associated proteinases have for the first time been studied and optimised in Lactobacillus delbrueckii subsp. lactis 313 (ATCC 7830; LDL 313). Concentrations of inoculum (0.1 < X < 10 % vol/vol), agitation speed (0 < S < 200 rpm), varying incubation temperature (30 < T < 50 °C), starting pH (4.5 < pH < 7) and carbon/nitrogen ratio of production medium (0.2 < r < 5) had an individual effect on proteinase yield (p < 0.01). Optimal conditions for proteinase production included an initial pH of 6.0, 45 °C incubation temperature, 2 % (v/v) inoculum size of OD560 = 1, 150 rpm agitation speed, and growth medium carbon/nitrogen ratio of 1.0. Maximum proteinase activity obtained for whole cells was 0.99 U/ml after 8 h of incubation. The variables studied are very relevant due to their significance in improving the productivity of proteinase synthesis from LDL 313, under process and, likely, economic optimum conditions.

dc.publisherHumana Press, Inc.
dc.titleOptimisation of batch culture conditions for cell-envelope-associated proteinase production from lactobacillus delbrueckii subsp. lactis ATCC® 7830™
dc.typeJournal Article
dcterms.source.volume168
dcterms.source.number5
dcterms.source.startPage1035
dcterms.source.endPage1050
dcterms.source.issn0273-2289
dcterms.source.titleApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
curtin.departmentCurtin Sarawak
curtin.accessStatusFulltext not available


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