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dc.contributor.authorChooi, Y.
dc.contributor.authorKrill, C.
dc.contributor.authorBarrow, R.
dc.contributor.authorChen, S.
dc.contributor.authorTrengove, R.
dc.contributor.authorOliver, Richard
dc.contributor.authorSolomon, P.
dc.date.accessioned2017-01-30T15:10:18Z
dc.date.available2017-01-30T15:10:18Z
dc.date.created2015-10-29T04:09:25Z
dc.date.issued2015
dc.date.submitted2015-10-29
dc.identifier.citationChooi, Y. and Krill, C. and Barrow, R. and Chen, S. and Trengove, R. and Oliver, R. and Solomon, P. 2015. An In planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum. Applied and Environmental Microbiology. 81 (1): pp. 177-186.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/43817
dc.identifier.doi10.1128/AEM.02745-14
dc.description.abstract

Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.

dc.publisherAmerican Society for Microbiology
dc.titleAn In planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum
dc.typeJournal Article
dcterms.dateSubmitted2015-10-29
dcterms.source.volume81
dcterms.source.number1
dcterms.source.startPage177
dcterms.source.endPage186
dcterms.source.issn0099-2240
dcterms.source.titleApplied and Environmental Microbiology
curtin.digitool.pid231640
curtin.pubStatusPublished
curtin.refereedTRUE
curtin.departmentCentre for Crop Disease Management
curtin.identifier.scriptidPUB-SE-DEA-RO-23433
curtin.identifier.elementsidELEMENTS-59980
curtin.accessStatusFulltext not available


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