Expressions of P-glycoprotein in treatment: Resistant Helicobacter pylori patients
MetadataShow full item record
Objective: There is an increasing incidence of Helicobacter pylori eradication therapy failure secondary to antibiotic resistance. In this study, we aimed to assess the P-glycoprotein expression levels among subjects who were H. pylori-positive and received multiple courses of eradication therapy to understand factors that may associate with their non-responses. Methods: Eleven resistant subjects were recruited during their hospital visit for upper gastrointestinal endoscopies. H. pylori infection status was confirmed by rapid urease test and bacterial culture. P-glycoprotein expressions from the antral and duodenal biopsies were measured by Western blot. The data was compared with H. pylori-negative (n = 54) and H. pylori-positive-treatment naïve (n = 22) that were recruited earlier in another study. Results: The resistant group showed higher relative antral P-glycoprotein expression com-pared to the H. pylori-negative group ( p = 0.0361). Most subjects demonstrated resistance to clarithromycin (72%), metronidazole (63.6%) or both (54.5%). No significant difference was observed between the P-glycoprotein expression in H. pylori-treatment resistant and H. pylori-positive-treatment naïve groups ( p = 0.319). Conclusion: H. pylori infection induces the expression of P-glycoprotein in the antrum. This study shows a potential protective mechanism which may be exploited through the use of P-glycoprotein inducers, such as rifabutin, may be beneficial in eradication regimens.
Showing items related by title, author, creator and subject.
P-glycoprotein expression in Helicobacter pylori-positive patients: The influence of MDR1 C3435T polymorphismOmar, Marhanis; Crowe, Andrew; Parsons, Richard; Ee, H.; Tay, C.; Hughes, Jeffery (2012)OBJECTIVE: The aim of this study was to determine whether the presence of Helicobacter pylori (H. pylori) infection and multidrug resistance protein 1 (MDR1) C3435T polymorphism had an influence on P-glycoprotein (P-gp) ...
Interrelationship between Heliobacter pylori and P-glycoprotein: potential role in drug resistance and carcinogenicityOmar, Marhanis Salihah (2013)The association between P-glycoprotein and Helicobacter pylori infection was explored. We found that P-glycoprotein expression is induced by H. pylori in the upper gastrointestinal tract and influenced by the genetic ...
Senarathna, Ganga; Crowe, Andrew (2015)Caco2 cells are a human adenocarcinoma cell line that forms tight junctions and are widely used to examine bidirectional drug transport as well as P-glycoprotein mediated efflux. Unfortunately Caco2 cell lines can be very ...