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    Innovative Microcapsules for Pancreatic ß-Cells Harvested from Mature Double-Transgenic Mice: Cell Imaging, Viability, Induced Glucose-Stimulated Insulin Measurements and Proinflammatory Cytokines Analysis

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    Fulltext not available
    Authors
    Mooranian, Armin
    Takechi, Ryu
    Jamieson, E.
    Morahan, G.
    Al-Salami, Hani
    Date
    2017
    Type
    Journal Article
    
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    Citation
    Mooranian, A. and Tackechi, R. and Jamieson, E. and Morahan, G. and Al-Salami, H. 2017. Innovative Microcapsules for Pancreatic ß-Cells Harvested from Mature Double-Transgenic Mice: Cell Imaging, Viability, Induced Glucose-Stimulated Insulin Measurements and Proinflammatory Cytokines Analysis. Pharmaceutical Research. 34 (6): pp. 1217-1223.
    Source Title
    Pharmaceutical Research
    DOI
    10.1007/s11095-017-2138-y
    ISSN
    0724-8741
    School
    School of Pharmacy
    URI
    http://hdl.handle.net/20.500.11937/51500
    Collection
    • Curtin Research Publications
    Abstract

    Purpose: Recently we demonstrated that microencapsulation of a murine pancreatic ß-cell line using an alginate-ursodeoxycholic acid (UDCA) matrix produced microcapsules with good stability and cell viability. In this study, we investigated if translation of this formulation to microencapsulation of primary ß-cells harvested from mature double-transgenic healthy mice would also generate stable microcapsules with good cell viability. Methods: Islets of Langerhans were isolated from Ngn3-GFP/RIP-DsRED mice by intraductal collagenase P digestion and density gradient centrifugation, dissociated into single cells and the ß-cell population purified by Fluorescence Activated Cell Sorting. ß-cells were microencapsulated using either alginate-poly-l-ornithine (F1; control) or alginate-poly-l-ornithine-UDCA (F2; test) formulations. Microcapsules were microscopically examined and microencapsulated cells were analyzed for viability, insulin and cytokine release, 2 days post-microencapsulation. Results: Microcapsules showed good uniformity and morphological characteristics and even cell distribution within microcapsules with or without UDCA. Two days post microencapsulation cell viability, mitochondrial ATP and insulin production were shown to be optimized in the presence of UDCA whilst production of the proinflammatory cytokine IL-1ß was reduced. Contradictory to our previous studies, UDCA did not reduce production of any other pro-inflammatory biomarkers. Conclusions: These results suggest that UDCA incorporation improves microcapsules’ physical and morphological characteristics and improves the viability and function of encapsulated mature primary pancreatic ß-cells.

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      BACKGROUND: In recent studies, we have incorporated bile acid and polyelectrolytes into pancreatic ß-cell microcapsules and examined their cell viability and microcapsule morphology. Cell viability remained low post ...
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      Mooranian, A.; Tackechi, R.; Jamieson, E.; Morahan, G.; Al-Salami, Hani (2018)
      Introduction: Ideal cell-containing microcapsules should be capable of maintaining cell viability and exhibit significant structural stability to support cellular functionality. To date, such microcapsules remain unavailable; ...
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