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    Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification

    Access Status
    Open access via publisher
    Authors
    Taillebois, L.
    Dudgeon, C.
    Maher, S.
    Crook, D.
    Saunders, T.
    Barton, D.
    Taylor, J.
    Welch, D.
    Newman, Stephen
    Travers, M.
    Saunders, R.
    Ovenden, J.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Taillebois, L. and Dudgeon, C. and Maher, S. and Crook, D. and Saunders, T. and Barton, D. and Taylor, J. et al. 2016. Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification. PeerJ. 2016 (8).
    Source Title
    PeerJ
    DOI
    10.7717/PEERJ.2418
    ISSN
    2167-8359
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/52360
    Collection
    • Curtin Research Publications
    Abstract

    © 2016 Taillebois et al.Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina MiSeq paired-end reads were used, with the latter demonstrating a higher quality of reads than the IonTorrent. From the 1-1.5 million raw reads per species, we successfully obtained 10-13 polymorphic loci for each species, which satisfied stringent design criteria. We developed multiplex panels for the amplification of the golden snapper and the blackspotted croaker loci, as well as post-amplification pooling panels for the grass emperor loci. The microsatellites characterized in this work were tested across three locations of northern Australia. The microsatellites we developed can detect population differentiation across northern Australia and may be used for genetic structure studies and stock identification.

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