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dc.contributor.authorKarajanagi, S.
dc.contributor.authorYoganathan, R.
dc.contributor.authorMammucari, R.
dc.contributor.authorPark, H.
dc.contributor.authorCox, J.
dc.contributor.authorZeitels, S.
dc.contributor.authorLanger, R.
dc.contributor.authorFoster, Neil
dc.identifier.citationKarajanagi, S. and Yoganathan, R. and Mammucari, R. and Park, H. and Cox, J. and Zeitels, S. and Langer, R. et al. 2011. Application of a dense gas technique for sterilizing soft biomaterials. Biotechnology and Bioengineering. 108 (7): pp. 1716-1725.

Sterilization of soft biomaterials such as hydrogels is challenging because existing methods such as gamma irradiation, steam sterilization, or ethylene oxide sterilization, while effective at achieving high sterility assurance levels (SAL), may compromise their physicochemical properties and biocompatibility. New methods that effectively sterilize soft biomaterials without compromising their properties are therefore required. In this report, a dense-carbon dioxide (CO 2 )-based technique was used to sterilize soft polyethylene glycol (PEG)-based hydrogels while retaining their structure and physicochemical properties. Conventional sterilization methods such as gamma irradiation and steam sterilization severely compromised the structure of the hydrogels. PEG hydrogels with high water content and low elastic shear modulus (a measure of stiffness) were deliberately inoculated with bacteria and spores and then subjected to dense CO 2 . The dense CO 2 -based methods effectively sterilized the hydrogels achieving a SAL of 10 -7 without compromising the viscoelastic properties, pH, water-content, and structure of the gels. Furthermore, dense CO 2 -treated gels were biocompatible and non-toxic when implanted subcutaneously in ferrets. The application of novel dense CO 2 -based methods to sterilize soft biomaterials has implications in developing safe sterilization methods for soft biomedical implants such as dermal fillers and viscosupplements. © 2011 Wiley Periodicals, Inc.

dc.publisherJohn Wiley & Sons
dc.titleApplication of a dense gas technique for sterilizing soft biomaterials
dc.typeJournal Article
dcterms.source.titleBiotechnology and Bioengineering
curtin.departmentDepartment of Chemical Engineering
curtin.accessStatusFulltext not available

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