Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Gene networks in skeletal muscle following endurance exercise are coexpressed in blood neutrophils and linked with blood inflammation markers

    Access Status
    Fulltext not available
    Authors
    Broadbent, J.
    Sampson, D.
    Sabapathy, S.
    Haseler, Luke
    Wagner, K.
    Bulmer, A.
    Peake, J.
    Neubauer, O.
    Date
    2017
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Broadbent, J. and Sampson, D. and Sabapathy, S. and Haseler, L. and Wagner, K. and Bulmer, A. and Peake, J. et al. 2017. Gene networks in skeletal muscle following endurance exercise are coexpressed in blood neutrophils and linked with blood inflammation markers. Journal of Applied Physiology. 122 (4): pp. 752-766.
    Source Title
    Journal of Applied Physiology
    DOI
    10.1152/japplphysiol.00114.2016
    ISSN
    8750-7587
    School
    School of Physiotherapy and Exercise Science
    URI
    http://hdl.handle.net/20.500.11937/57289
    Collection
    • Curtin Research Publications
    Abstract

    Copyright © 2017 the American Physiological Society. It remains incompletely understood whether there is an association between the transcriptome profiles of skeletal muscle and blood leukocytes in response to exercise or other physiological stressors. We have previously analyzed the changes in the muscle and blood neutrophil transcriptome in eight trained men before and 3, 48, and 96 h after 2 h cycling and running. Because we collected muscle and blood in the same individuals and under the same conditions, we were able to directly compare gene expression between the muscle and blood neutrophils. Applying weighted gene coexpression network analysis (WGCNA) as an advanced network-driven method to these original data sets enabled us to compare the muscle and neutrophil transcriptomes in a rigorous and systematic manner. Two gene networks were identified th at were preserved between skeletal muscle and blood neutrophils, functionally related to mitochondria and posttranslational processes. Strong preservation measures (Z summary > 10) for both muscle-neutrophil gene networks were evident within the postexercise recovery period. Muscle and neutrophil gene coexpression was strongly correlated in the mitochondria-related network (r = 0.97; P = 3.17E-2). We also identified multiple correlations between muscular gene subnetworks and exercise-induced changes in blood leukocyte counts, inflammation, and muscle damage markers. These data reveal previously unidentified gene coexpression between skeletal muscle and blood neutrophils following exercise, showing the value of WGCNA to understand exercise physiology. Furthermore, these findings provide preliminary evidence in support of the notion that blood neutrophil gene networks may potentially help us to track physiological and pathophysiological changes in the muscle.

    Related items

    Showing items related by title, author, creator and subject.

    • Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress
      Neubauer, O.; Sabapathy, S.; Lazarus, R.; Jowett, J.; Desbrow, B.; Peake, J.; Cameron-Smith, D.; Haseler, Luke; Wagner, K.; Bulmer, A. (2013)
      Neutrophils serve as an intriguing model for the study of innate immune cellular activity induced by physiological stress. We measured changes in the transcriptome of circulating neutrophils following an experimental ...
    • Time course-dependent changes in the transcriptome of human skeletal muscle during recovery from endurance exercise: From inflammation to adaptive remodeling
      Neubauer, O.; Sabapathy, S.; Ashton, K.; Desbrow, B.; Peake, J.; Lazarus, R.; Wessner, B.; Cameron-Smith, D.; Wagner, K.; Haseler, Luke; Bulmer, A. (2014)
      Reprogramming of gene expression is fundamental for skeletal muscle adaptations in response to endurance exercise. This study investigated the time course-dependent changes in the muscular transcriptome after an endurance ...
    • Is the soleus a sentinel muscle for impaired aerobic capacity in heart failure?
      Panizzolo, F.; Maiorana, Andrew; Naylor, L.; Lichtwark, G.; Dembo, L.; Lloyd, D.; Green, D.; Rubenson, J. (2015)
      Purpose: Skeletal muscle wasting is well documented in chronic heart failure (CHF). This article provides a more detailed understanding of the morphology behind this muscle wasting and the relation between muscle morphology, ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.