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dc.contributor.authorNeubauer, O.
dc.contributor.authorSabapathy, S.
dc.contributor.authorAshton, K.
dc.contributor.authorDesbrow, B.
dc.contributor.authorPeake, J.
dc.contributor.authorLazarus, R.
dc.contributor.authorWessner, B.
dc.contributor.authorCameron-Smith, D.
dc.contributor.authorWagner, K.
dc.contributor.authorHaseler, Luke
dc.contributor.authorBulmer, A.
dc.identifier.citationNeubauer, O. and Sabapathy, S. and Ashton, K. and Desbrow, B. and Peake, J. and Lazarus, R. and Wessner, B. et al. 2014. Time course-dependent changes in the transcriptome of human skeletal muscle during recovery from endurance exercise: From inflammation to adaptive remodeling. Journal of Applied Physiology. 116 (3): pp. 274-287.

Reprogramming of gene expression is fundamental for skeletal muscle adaptations in response to endurance exercise. This study investigated the time course-dependent changes in the muscular transcriptome after an endurance exercise trial consisting of 1 h of intense cycling immediately followed by 1 h of intense running. Skeletal muscle samples were taken at baseline, 3 h, 48 h, and 96 h postexercise from eight healthy, endurance-trained men. RNA was extracted from muscle. Differential gene expression was evaluated using Illumina microarrays and validated with qPCR. Gene set enrichment analysis identified enriched molecular signatures chosen from the Molecular Signatures Database. Three hours postexercise, 102 gene sets were upregulated [family wise error rate (FWER), P < 0.05], including groups of genes related with leukocyte migration, immune and chaperone activation, and cyclic AMP responsive element binding protein (CREB) 1 signaling. Forty-eight hours postexercise, among 19 enriched gene sets (FWER, P < 0.05), two gene sets related to actin cytoskeleton remodeling were upregulated. Ninety-six hours postexercise, 83 gene sets were enriched (FWER, P < 0.05), 80 of which were upregulated, including gene groups related to chemokine signaling, cell stress management, and extracellular matrix remodeling. These data provide comprehensive insights into the molecular pathways involved in acute stress, recovery, and adaptive muscular responses to endurance exercise. The novel 96 h postexercise transcriptome indicates substantial transcriptional activity potentially associated with the prolonged presence of leukocytes in the muscles. This suggests that muscular recovery, from a transcriptional perspective, is incomplete 96 h after endurance exercise involving muscle damage. Copyright © 2014 the American Physiological Society.

dc.publisherThe American Physiological Society
dc.titleTime course-dependent changes in the transcriptome of human skeletal muscle during recovery from endurance exercise: From inflammation to adaptive remodeling
dc.typeJournal Article
dcterms.source.titleJournal of Applied Physiology
curtin.departmentSchool of Physiotherapy and Exercise Science
curtin.accessStatusOpen access via publisher

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