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Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia

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Open access via publisher
Authors
Mulroney, K.
Hall, J.
Huang, X.
Turnbull, E.
Bzdyl, N.
Chakera, Aron
Naseer, U.
Corea, E.
Ellington, M.
Hopkins, K.
Wester, A.
Ekelund, O.
Woodford, N.
Inglis, T.
Date
2017
Type
Journal Article

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Citation
Mulroney, K. and Hall, J. and Huang, X. and Turnbull, E. and Bzdyl, N. and Chakera, A. and Naseer, U. et al. 2017. Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia. Scientific Reports. 7 (1).
Source Title
Scientific Reports
DOI
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School
Curtin Medical School
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Abstract

© 2017 The Author(s). The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MIC FAST ) correspond closely with broth microdilution MIC (MIC BMD , Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.